1ahs: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1ahs.gif|left|200px]] | [[Image:1ahs.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1ahs", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
| | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| | or leave the SCENE parameter empty for the default display. | ||
--> | |||
{{STRUCTURE_1ahs| PDB=1ahs | SCENE= }} | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7''' | '''CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7''' | ||
| Line 27: | Line 24: | ||
[[Category: Gouet, P.]] | [[Category: Gouet, P.]] | ||
[[Category: Stuart, D.]] | [[Category: Stuart, D.]] | ||
[[Category: | [[Category: Core protein]] | ||
[[Category: | [[Category: Glycoprotein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:17:12 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 10:17, 2 May 2008
CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7
OverviewOverview
The baculovirus-expressed core protein VP7 of African horse sickness virus serotype 4 (AHSV-4) has been purified to homogeneity and crystallized in the presence of 2.8 M urea. The X-ray structure has been solved to a 2.3-Angstroms (1 Angstrom = 0.1 nm) resolution with an Rfactor of 19.8%. The structure of AHSV VP7 reveals that during crystallization, the two-domain protein is cleaved and only the top domain remains. A similar problem was encountered previously with bluetongue virus (BTV) VP7 (whose structure has been reported), showing that the connections between the top and the bottom domains are rather weak for these two distinct orbiviruses. The top domains of both BTV and AHSV VP7 are trimeric and structurally very similar. The electron density maps show that they both possess an extra electron density feature along their molecular threefold axes, which is most likely due to an unidentified ion. The characteristics of the molecular surface of BTV and AHSV VP7 suggest why AHSV VP7 is much less soluble than BTV VP7 and indicate the possibility of attachment to the cell via attachment of an Arg-Gly-Asp (RGD) motif in the top domain of VP7 to a cellular integrin for both of these orbiviruses.
About this StructureAbout this Structure
1AHS is a Single protein structure of sequence from African horsesickness virus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the top domain of African horse sickness virus VP7: comparisons with bluetongue virus VP7., Basak AK, Gouet P, Grimes J, Roy P, Stuart D, J Virol. 1996 Jun;70(6):3797-806. PMID:8648715 Page seeded by OCA on Fri May 2 10:17:12 2008