User:Nikhil Malvankar/Geobacter pilus structure and function: Difference between revisions
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Our electron cryomicroscopic structure of ''Geobacter sulfurreducens'' pili (<scene name='83/834714/Filament/4'>restore initial scene</scene>), [[6vk9]], reveals them to be <scene name='83/834714/Filament/1'>composed of a core</scene> of '''<font color='#e87000'>PilA-N</font>''' (61 amino acids) coated with an outer surface layer of '''<font color='00a0a0'>PilA-C</font>''' (104 amino acids). Here is a <scene name='83/834714/Filament/2'>cutaway view</scene> (front half hidden). The C-termini of '''<font color='#e87000'>PilA-N</font>''' <scene name='83/834714/Filament/3'>protrude into sockets</scene> in '''<font color='00a0a0'>PilA-C</font>'''. | Our electron cryomicroscopic structure of ''Geobacter sulfurreducens'' pili (<scene name='83/834714/Filament/4'>restore initial scene</scene>), [[6vk9]], reveals them to be <scene name='83/834714/Filament/1'>composed of a core</scene> of '''<font color='#e87000'>PilA-N</font>''' (61 amino acids) coated with an outer surface layer of '''<font color='00a0a0'>PilA-C</font>''' (104 amino acids). Here is a <scene name='83/834714/Filament/2'>cutaway view</scene> (front half hidden). The C-termini of '''<font color='#e87000'>PilA-N</font>''' <scene name='83/834714/Filament/3'>protrude into sockets</scene> in '''<font color='00a0a0'>PilA-C</font>'''. | ||
The '''PilA-N''' subunits have extensive hydrophobic contacts with each other, stabilizing the hydrophobic core of the filament. <scene name='83/834714/Filament/5'>View PilA-N with PilA-C hidden</scene>. Each PilA-N chain contacts 75 carbon atoms from 11 adjacent PilA-N chains, and also has 4 hydrogen bonds and 4 salt bridges with adjacent PilA-N chains (not shown). In contrast, '''PilA-C''' subunits (<scene name='83/834714/Filament/6'>view PilA-C with front half and PilA-N hidden</scene>) have little contact with each other: 14 atoms, which are mostly hydrogen bonded, with one salt bridge. | The '''PilA-N''' subunits have extensive hydrophobic contacts with each other, stabilizing the hydrophobic core of the filament. <scene name='83/834714/Filament/5'>View PilA-N with PilA-C hidden</scene>. Each PilA-N chain contacts 75 carbon atoms from 11 adjacent PilA-N chains, and also has 4 hydrogen bonds and 4 salt bridges with adjacent PilA-N chains (not shown). In contrast, '''PilA-C''' subunits (<scene name='83/834714/Filament/6'>view PilA-C with front half and PilA-N hidden</scene>) have little contact with each other: 14 atoms, which are mostly hydrogen bonded, with one salt bridge (not shown). | ||
===Heterodimers=== | ===Heterodimers=== | ||