4ay1: Difference between revisions
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==Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide binding properties== | ==Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide binding properties== | ||
<StructureSection load='4ay1' size='340' side='right' caption='[[4ay1]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='4ay1' size='340' side='right'caption='[[4ay1]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ay1]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AY1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AY1 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4ay1]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AY1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AY1 FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ay1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ay1 OCA], [http://pdbe.org/4ay1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ay1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ay1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ay1 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ay1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ay1 OCA], [http://pdbe.org/4ay1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ay1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ay1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ay1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/CH3L2_HUMAN CH3L2_HUMAN]] Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.<ref>PMID:22742450</ref> | [[http://www.uniprot.org/uniprot/CH3L2_HUMAN CH3L2_HUMAN]] Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.<ref>PMID:22742450</ref> | ||
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==See Also== | ==See Also== | ||
*[[Chitinase-3-like protein|Chitinase-3-like protein]] | *[[Chitinase-3-like protein 3D structures|Chitinase-3-like protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
[[Category: Large Structures]] | |||
[[Category: Aalten, D M.F van]] | [[Category: Aalten, D M.F van]] | ||
[[Category: Betou, M]] | [[Category: Betou, M]] | ||
Revision as of 19:40, 29 January 2020
Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide binding propertiesHuman YKL-39 is a pseudo-chitinase with retained chitooligosaccharide binding properties
Structural highlights
Function[CH3L2_HUMAN] Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.[1] Publication Abstract from PubMedThe chitinase-like proteins YKL-39 (chitinase 3-like-2) and YKL-40 (chitinase 3-like-1) are highly expressed in a number of human cells independent of their origin (mesenchymal, epithelial or haemapoietic). Elevated serum levels of YKL-40 have been associated with a negative outcome in a number of diseases ranging from cancer to inflammation and asthma. YKL-39 expression has been associated with osteoarthritis. However, despite the reported association with disease, the physiological or pathological role of these proteins is still very poorly understood. Although YKL-39 is homologous to the two family 18 chitinases in the human genome, it has been reported to lack any chitinase activity. In the present study, we show that human YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. A glycan screen identified oligomers of N-acetylglucosamine as preferred binding partners. YKL-39 binds chitooligosaccharides and a newly synthesized derivative of the bisdionin chitinase-inhibitor class with micromolar affinity, through a number of conserved tryptophan residues. Strikingly, the chitinase activity of YKL-39 was recovered by reverting two non-conservative substitutions in the active site to those found in the active enzymes, suggesting that YKL-39 is a pseudo-chitinase with retention of chitinase-like ligand-binding properties. Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide-binding properties.,Schimpl M, Rush CL, Betou M, Eggleston IM, Recklies AD, van Aalten DM Biochem J. 2012 Aug 15;446(1):149-57. PMID:22742450[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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