1aew: Difference between revisions

Revision as of 10:10, 2 May 2008

L-CHAIN HORSE APOFERRITIN

OverviewOverview

Mammalian ferritins are 24-mers assembled from two types of polypeptide chain which provide the molecule with different functions. H(eavy) chains catalyse the first step in iron storage, the oxidation of iron(II). L(ight) chains promote the nucleation of the mineral ferrihydrite enabling storage of iron(III) inside the protein shell. We report here the comparison of the three-dimensional structures of recombinant human H chain (HuHF) and horse L chain (HoLF) ferritin homopolymers, which have been refined at 1.9 A resolution. There is 53% sequence identity between these molecules, and the two structures are very similar, the H and L subunit alpha-carbons superposing to within 0.5 A rms deviation with 41 water molecules in common. Nevertheless, there are significant important differences which can be related to differences in function. In particular, the centres of the four-helix bundles contain distinctive groups of hydrophilic residues which have been associated with ferroxidase activity in H chains and enhanced stability in L chains. L chains contain a group of glutamates associated with mineralisation within the iron storage cavity of the protein.

About this StructureAbout this Structure

1AEW is a Single protein structure of sequence from Equus caballus. The following page contains interesting information on the relation of 1AEW with [Ferritin and Transferrin]. Full crystallographic information is available from OCA.

ReferenceReference

Comparison of the three-dimensional structures of recombinant human H and horse L ferritins at high resolution., Hempstead PD, Yewdall SJ, Fernie AR, Lawson DM, Artymiuk PJ, Rice DW, Ford GC, Harrison PM, J Mol Biol. 1997 May 2;268(2):424-48. PMID:9159481 Page seeded by OCA on Fri May 2 10:10:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1aew.gif|left|200px]]
- {{Structure
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-}}
 '''L-CHAIN HORSE APOFERRITIN'''
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 [[Category: Lawson, D M.]]
 [[Category: Yewdall, S J.]]
-[[Category: acetylation]]
+[[Category: Acetylation]]
-[[Category: iron storage]]
+[[Category: Iron storage]]
-[[Category: multigene family]]
+[[Category: Multigene family]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:10:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:38:58 2008''