1wc3: Difference between revisions
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==Soluble adenylyl cyclase CyaC from S. platensis in complex with alpha, beta-methylene-ATP and Strontium== | ==Soluble adenylyl cyclase CyaC from S. platensis in complex with alpha, beta-methylene-ATP and Strontium== | ||
<StructureSection load='1wc3' size='340' side='right' caption='[[1wc3]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='1wc3' size='340' side='right'caption='[[1wc3]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1wc3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Artpt Artpt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WC3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WC3 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1wc3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Artpt Artpt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WC3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WC3 FirstGlance]. <br> | ||
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==See Also== | ==See Also== | ||
*[[Adenylyl cyclase|Adenylyl cyclase]] | *[[3D Adenylyl cyclase 3D structures|3D Adenylyl cyclase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Adenylate cyclase]] | [[Category: Adenylate cyclase]] | ||
[[Category: Artpt]] | [[Category: Artpt]] | ||
[[Category: Large Structures]] | |||
[[Category: Buck, J]] | [[Category: Buck, J]] | ||
[[Category: Levin, L R]] | [[Category: Levin, L R]] | ||
Revision as of 20:23, 22 January 2020
Soluble adenylyl cyclase CyaC from S. platensis in complex with alpha, beta-methylene-ATP and StrontiumSoluble adenylyl cyclase CyaC from S. platensis in complex with alpha, beta-methylene-ATP and Strontium
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedIn an evolutionarily conserved signaling pathway, 'soluble' adenylyl cyclases (sACs) synthesize the ubiquitous second messenger cyclic adenosine 3',5'-monophosphate (cAMP) in response to bicarbonate and calcium signals. Here, we present crystal structures of a cyanobacterial sAC enzyme in complex with ATP analogs, calcium and bicarbonate, which represent distinct catalytic states of the enzyme. The structures reveal that calcium occupies the first ion-binding site and directly mediates nucleotide binding. The single ion-occupied, nucleotide-bound state defines a novel, open adenylyl cyclase state. In contrast, bicarbonate increases the catalytic rate by inducing marked active site closure and recruiting a second, catalytic ion. The phosphates of the bound substrate analogs are rearranged, which would facilitate product formation and release. The mechanisms of calcium and bicarbonate sensing define a reaction pathway involving active site closure and metal recruitment that may be universal for class III cyclases. Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment.,Steegborn C, Litvin TN, Levin LR, Buck J, Wu H Nat Struct Mol Biol. 2005 Jan;12(1):32-7. Epub 2004 Dec 26. PMID:15619637[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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