Sandbox Reserved 1105: Difference between revisions
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TTR is a specific carrier of RBP ([[1ggl]]). RBPs have a molecular mass of 21 kDa. They are composed of an eight-stranded β-barrel and a C-terminal α-helix. | TTR is a specific carrier of RBP ([[1ggl]]). RBP are specific carriers circulating in complex with TTR in the plasma. It transports vitamin A (retinol), mainly synthesized in the liver, to its final tissue, the carrier is needed fo retinol secretion<ref name= "Naylor"/>. Also, they are markers for acute undernutrition. For instance, it is associated to insulin-resistance for type 2 diabetes. RBP concentration in the serum allows to diagnose several diseases. | ||
RBPs have a molecular mass of 21 kDa. They are composed of an eight-stranded β-barrel and a C-terminal α-helix. | |||
One tetramer of TTR can bind two molecules of RBP in vitro (1:2 stoichiometry). However, when we isolate the TTR-RBP complex from the plasma (in vivo) we find a 1:1 stoichiometry <ref name= "Naylor"> "Naylor, H. M., & Newcomer, M. E. (1999). The Structure of Human Retinol-Binding Protein (RBP) with Its Carrier Protein Transthyretin Reveals an Interaction with the Carboxy Terminus of RBP†,‡. Biochemistry, 38(9), 2647–2653. doi:http://dx.doi.org/10.1021/bi982291i"</ref> . | One tetramer of TTR can bind two molecules of RBP in vitro (1:2 stoichiometry). However, when we isolate the TTR-RBP complex from the plasma (in vivo) we find a 1:1 stoichiometry <ref name= "Naylor"> "Naylor, H. M., & Newcomer, M. E. (1999). The Structure of Human Retinol-Binding Protein (RBP) with Its Carrier Protein Transthyretin Reveals an Interaction with the Carboxy Terminus of RBP†,‡. Biochemistry, 38(9), 2647–2653. doi:http://dx.doi.org/10.1021/bi982291i"</ref> . | ||