1aa4: Difference between revisions

Revision as of 10:02, 2 May 2008

SPECIFICITY OF LIGAND BINDING IN A BURIED POLAR CAVITY OF CYTOCHROME C PEROXIDASE

OverviewOverview

Conformational changes that gate the access of substrates or ligands to an active site are important features of enzyme function. In this report, we describe an unusual example of a structural rearrangement near a buried artificial cavity in cytochrome c peroxidase that occurs on binding protonated benzimidazole. A hinged main-chain rotation at two residues (Pro 190 and Asn 195) results in a surface loop rearrangement that opens a large solvent-accessible channel for the entry of ligands to an otherwise inaccessible binding site. The trapping of this alternate conformational state provides a unique view of the extent to which protein dynamics can allow small molecule penetration into buried protein cavities.

About this StructureAbout this Structure

1AA4 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

A ligand-gated, hinged loop rearrangement opens a channel to a buried artificial protein cavity., Fitzgerald MM, Musah RA, McRee DE, Goodin DB, Nat Struct Biol. 1996 Jul;3(7):626-31. PMID:8673607 Page seeded by OCA on Fri May 2 10:02:07 2008

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OCA

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 [[Image:1aa4.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1aa4 |SIZE=350|CAPTION= <scene name='initialview01'>1aa4</scene>, resolution 2.1&Aring;
+The line below this paragraph, containing "STRUCTURE_1aa4", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=ACT:Removal+Of+TRP+191+Forms+An+Internal+Cavity+Below+The+He+...'>ACT</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= CCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_1aa4|  PDB=1aa4  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aa4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aa4 OCA], [http://www.ebi.ac.uk/pdbsum/1aa4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1aa4 RCSB]</span>
-}}
 '''SPECIFICITY OF LIGAND BINDING IN A BURIED POLAR CAVITY OF CYTOCHROME C PEROXIDASE'''
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 [[Category: Mcree, D E.]]
 [[Category: Musah, R A.]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: peroxidase]]
+[[Category: Peroxidase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:02:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:36:32 2008''