6drv: Difference between revisions
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==Beta-galactosidase== | ==Beta-galactosidase== | ||
<StructureSection load='6drv' size='340' side='right' caption='[[6drv]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='6drv' size='340' side='right'caption='[[6drv]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6drv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DRV FirstGlance]. <br> | <table><tr><td colspan='2'>[[6drv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DRV FirstGlance]. <br> | ||
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[[Category: Beta-galactosidase]] | [[Category: Beta-galactosidase]] | ||
[[Category: Ecoli]] | [[Category: Ecoli]] | ||
[[Category: Large Structures]] | |||
[[Category: Cianfrocco, M A]] | [[Category: Cianfrocco, M A]] | ||
[[Category: DiMaio, F]] | [[Category: DiMaio, F]] | ||
Revision as of 11:54, 8 January 2020
Beta-galactosidaseBeta-galactosidase
Structural highlights
Publication Abstract from PubMedAccess to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2A structure of ss-galactosidase in approximately 55h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud. cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud.,Cianfrocco MA, Lahiri I, DiMaio F, Leschziner AE J Struct Biol. 2018 Jun 1. pii: S1047-8477(18)30138-2. doi:, 10.1016/j.jsb.2018.05.014. PMID:29864529[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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