5uvr: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==The core region of PilO from the type IV pilus system of Pseudomonas aeruginosa== | ==The core region of PilO from the type IV pilus system of Pseudomonas aeruginosa== | ||
<StructureSection load='5uvr' size='340' side='right' caption='[[5uvr]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='5uvr' size='340' side='right'caption='[[5uvr]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5uvr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UVR FirstGlance]. <br> | <table><tr><td colspan='2'>[[5uvr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UVR FirstGlance]. <br> | ||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pilO, PAERUG_E15_London_28_01_14_03389, PAERUG_P32_London_17_VIM_2_10_11_01500 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uvr OCA], [http://pdbe.org/5uvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uvr RCSB], [http://www.ebi.ac.uk/pdbsum/5uvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uvr ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uvr OCA], [http://pdbe.org/5uvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uvr RCSB], [http://www.ebi.ac.uk/pdbsum/5uvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uvr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| Line 20: | Line 21: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Howell, P L]] | [[Category: Howell, P L]] | ||
[[Category: Junop, M S]] | [[Category: Junop, M S]] | ||
Revision as of 11:23, 8 January 2020
The core region of PilO from the type IV pilus system of Pseudomonas aeruginosaThe core region of PilO from the type IV pilus system of Pseudomonas aeruginosa
Structural highlights
Publication Abstract from PubMedPseudomonas aeruginosa uses long, thin fibres called type IV pili (T4P) for adherence to surfaces, biofilm formation, and twitching motility. A conserved subcomplex of PilMNOP is required for extension and retraction of T4P. To better understand its function, we attempted to co-crystallize the soluble periplasmic portions of PilNOP, using reductive surface methylation to promote crystal formation. Only PilODelta109 crystallized; its structure was determined to 1.7 A resolution using molecular replacement. This new structure revealed two novel features: a shorter N-terminal alpha1-helix followed by a longer unstructured loop, and a discontinuous beta-strand in the second alphabetabeta motif, mirroring that in the first motif. PISA analysis identified a potential dimer interface with striking similarity to that of the PilO homolog EpsM from the Vibrio cholerae type II secretion system. We identified highly conserved residues within predicted unstructured regions in PilO proteins from various Pseudomonads and performed site-directed mutagenesis to assess their role in T4P function. R169D and I170A substitutions decreased surface piliation and twitching motility without disrupting PilO homodimer formation. These residues could form important protein-protein interactions with PilN or PilP. This work furthers our understanding of residues critical for T4aP function. Conserved, unstructured regions in Pseudomonas aeruginosa PilO are important for type IVa pilus function.,Leighton TL, Mok MC, Junop MS, Howell PL, Burrows LL Sci Rep. 2018 Feb 8;8(1):2600. doi: 10.1038/s41598-018-20925-w. PMID:29422606[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||