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'''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS''' | '''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS''' | ||
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[[Category: Hasnain, S S.]] | [[Category: Hasnain, S S.]] | ||
[[Category: Ingledew, W J.]] | [[Category: Ingledew, W J.]] | ||
[[Category: | [[Category: Copper protein]] | ||
[[Category: | [[Category: Direct method]] | ||
[[Category: | [[Category: Electron transport]] | ||
[[Category: | [[Category: Medium resolution]] | ||
[[Category: | [[Category: Metalloprotein]] | ||
[[Category: | [[Category: Sa]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:59:39 2008'' | |||
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Revision as of 09:59, 2 May 2008
STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS
OverviewOverview
The structure of rusticyanin, an acid-stable copper protein, has been determined at 2.1 A resolution by direct methods combined with the single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the largest unknown protein structure (Mr approximately /= 16.8 kDa) to be determined using the SAS and direct-methods approach and demonstrates that by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at typical (approximately 2 A) macromolecular crystallographic resolutions. Extrapolating from the size of the anomalous signal for copper (f" approximately 4 e-), this result suggests that the approach could be used for proteins with molecular weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80 kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The method provides a powerful alternative in solving a de novo protein structure without either preparing multiple crystals (i.e. isomorphous heavy-atom derivative plus native crystals) or collecting multi-wavelength anomalous diffraction (MAD) data.
About this StructureAbout this Structure
1A8Z is a Single protein structure of sequence from Acidithiobacillus ferrooxidans. Full crystallographic information is available from OCA.
ReferenceReference
Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:9761859 Page seeded by OCA on Fri May 2 09:59:39 2008