6pbd: Difference between revisions
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<StructureSection load='6pbd' size='340' side='right'caption='[[6pbd]], [[Resolution|resolution]] 2.34Å' scene=''> | <StructureSection load='6pbd' size='340' side='right'caption='[[6pbd]], [[Resolution|resolution]] 2.34Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6pbd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PBD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PBD FirstGlance]. <br> | <table><tr><td colspan='2'>[[6pbd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15252_[[caulobacter_crescentus_poindexter_1964]] Atcc 15252 [[caulobacter crescentus poindexter 1964]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PBD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PBD FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ccrMIM, ccrM, CC_0378 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155892 ATCC 15252 [[Caulobacter crescentus Poindexter 1964]]])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pbd OCA], [http://pdbe.org/6pbd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pbd RCSB], [http://www.ebi.ac.uk/pdbsum/6pbd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pbd ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pbd OCA], [http://pdbe.org/6pbd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pbd RCSB], [http://www.ebi.ac.uk/pdbsum/6pbd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pbd ProSAT]</span></td></tr> | ||
Revision as of 15:04, 1 January 2020
DNA N6-Adenine Methyltransferase CcrM In Complex with Double-Stranded DNA Oligonucleotide Containing Its Recognition Sequence GAATCDNA N6-Adenine Methyltransferase CcrM In Complex with Double-Stranded DNA Oligonucleotide Containing Its Recognition Sequence GAATC
Structural highlights
Function[MTC1_CAUVC] This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Publication Abstract from PubMedThe Caulobacter crescentus cell cycle-regulated DNA methyltransferase (CcrM) methylates the adenine of hemimethylated GANTC after replication. Here we present the structure of CcrM in complex with double-stranded DNA containing the recognition sequence. CcrM contains an N-terminal methyltransferase domain and a C-terminal nonspecific DNA-binding domain. CcrM is a dimer, with each monomer contacting primarily one DNA strand: the methyltransferase domain of one molecule binds the target strand, recognizes the target sequence, and catalyzes methyl transfer, while the C-terminal domain of the second molecule binds the non-target strand. The DNA contacts at the 5-base pair recognition site results in dramatic DNA distortions including bending, unwinding and base flipping. The two DNA strands are pulled apart, creating a bubble comprising four recognized base pairs. The five bases of the target strand are recognized meticulously by stacking contacts, van der Waals interactions and specific Watson-Crick polar hydrogen bonds to ensure high enzymatic specificity. The cell cycle-regulated DNA adenine methyltransferase CcrM opens a bubble at its DNA recognition site.,Horton JR, Woodcock CB, Opot SB, Reich NO, Zhang X, Cheng X Nat Commun. 2019 Oct 10;10(1):4600. doi: 10.1038/s41467-019-12498-7. PMID:31601797[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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