6mr6: Difference between revisions
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==E. coli cysteine desulfurase SufS H55A with a cysteine persulfide intermediate== | ==E. coli cysteine desulfurase SufS H55A with a cysteine persulfide intermediate== | ||
<StructureSection load='6mr6' size='340' side='right' caption='[[6mr6]], [[Resolution|resolution]] 2.02Å' scene=''> | <StructureSection load='6mr6' size='340' side='right'caption='[[6mr6]], [[Resolution|resolution]] 2.02Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6mr6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MR6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MR6 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6mr6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MR6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MR6 FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 6mr6" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6mr6" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Cysteine desulfurase 3D structures|Cysteine desulfurase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Ecoli]] | [[Category: Ecoli]] | ||
[[Category: Large Structures]] | |||
[[Category: Dunkle, J A]] | [[Category: Dunkle, J A]] | ||
[[Category: Frantom, P A]] | [[Category: Frantom, P A]] | ||
Revision as of 14:28, 1 January 2020
E. coli cysteine desulfurase SufS H55A with a cysteine persulfide intermediateE. coli cysteine desulfurase SufS H55A with a cysteine persulfide intermediate
Structural highlights
Function[SUFS_ECOLI] Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L-selenocysteine. Selenocysteine lyase activity is however unsure in vivo.[1] [2] [3] [4] [5] Publication Abstract from PubMedSufS is a type II cysteine desulfurase and acts as the initial step in the Suf Fe-S cluster assembly pathway. In Escherichia coli this pathway is utilized under conditions of oxidative stress and is resistant to reactive oxygen species. Mechanistically this means SufS must shift between protecting a covalent persulfide intermediate and making it available for transfer to the next protein partner in the pathway, SufE. Here, we report five x-ray crystal structures of SufS including a new structure of SufS containing an inward facing persulfide intermediate on C364. Additional structures of SufS variants with substitutions at the dimer interface show changes in dimer geometry and suggest a conserved beta-hairpin structure plays a role in mediating interactions with SufE. These new structures, along with previous HDX-MS and biochemical data identify an interaction network capable of communication between active-sites of the SufS dimer coordinating the shift between desulfurase and transpersulfurase activities. Structural evidence for dimer-interface driven regulation of the type II cysteine desulfurase, SufS.,Dunkle JA, Bruno M, Outten FW, Frantom PA Biochemistry. 2018 Dec 20. doi: 10.1021/acs.biochem.8b01122. PMID:30571100[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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