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==Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)== | ==Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)== | ||
<StructureSection load='6d38' size='340' side='right' caption='[[6d38]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='6d38' size='340' side='right'caption='[[6d38]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6d38]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D38 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6d38]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D38 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6d39|6d39]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6d39|6d39]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d38 OCA], [http://pdbe.org/6d38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d38 RCSB], [http://www.ebi.ac.uk/pdbsum/6d38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d38 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d38 OCA], [http://pdbe.org/6d38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d38 RCSB], [http://www.ebi.ac.uk/pdbsum/6d38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d38 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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</div> | </div> | ||
<div class="pdbe-citations 6d38" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6d38" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Dronpa|Dronpa]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Echinophyllia sp. sc22]] | |||
[[Category: Large Structures]] | |||
[[Category: Fan, L]] | [[Category: Fan, L]] | ||
[[Category: Lin, M]] | [[Category: Lin, M]] | ||
Revision as of 13:55, 1 January 2020
Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)
Structural highlights
Publication Abstract from PubMedProtein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light. Optical control of cell signaling by single-chain photoswitchable kinases.,Zhou XX, Fan LZ, Li P, Shen K, Lin MZ Science. 2017 Feb 24;355(6327):836-842. doi: 10.1126/science.aah3605. PMID:28232577[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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