5via: Difference between revisions
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==Crystal structural of Leishmania major pseudoperoxidase== | ==Crystal structural of Leishmania major pseudoperoxidase== | ||
<StructureSection load='5via' size='340' side='right' caption='[[5via]], [[Resolution|resolution]] 1.76Å' scene=''> | <StructureSection load='5via' size='340' side='right'caption='[[5via]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5via]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VIA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VIA FirstGlance]. <br> | <table><tr><td colspan='2'>[[5via]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VIA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VIA FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LMJF_21_1567 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 LEIMA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5via FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5via OCA], [http://pdbe.org/5via PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5via RCSB], [http://www.ebi.ac.uk/pdbsum/5via PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5via ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5via FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5via OCA], [http://pdbe.org/5via PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5via RCSB], [http://www.ebi.ac.uk/pdbsum/5via PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5via ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Leima]] | |||
[[Category: Chreifi, G]] | [[Category: Chreifi, G]] | ||
[[Category: Dejam, D]] | [[Category: Dejam, D]] | ||
Revision as of 12:34, 1 January 2020
Crystal structural of Leishmania major pseudoperoxidaseCrystal structural of Leishmania major pseudoperoxidase
Structural highlights
Publication Abstract from PubMedLeishmania major pseudoperoxidase (LmPP) is a recently discovered heme protein expressed by the human pathogen. Previous in vivo and in vitro studies suggest that LmPP is a crucial element of the pathogen's defense mechanism against the reactive nitrogen species peroxynitrite produced during the host immune response. To shed light on the potential mechanism of peroxynitrite detoxification, we have determined the 1.76-A X-ray crystal structure of LmPP, revealing a striking degree of homology with heme peroxidases. The most outstanding structural feature is a Cys/His heme coordination, which corroborates previous spectroscopic and mutagenesis studies. We also used a combination of stopped-flow and electron paramagnetic spectroscopies that together suggest that peroxynitrite is not a substrate for LmPP catalysis, leaving the function of LmPP an open question. Crystal structure and functional analysis of Leishmania major pseudoperoxidase.,Chreifi G, Dejam D, Poulos TL J Biol Inorg Chem. 2017 Aug;22(6):919-927. doi: 10.1007/s00775-017-1469-9. Epub, 2017 Jun 5. PMID:28584975[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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