5uf6: Difference between revisions
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==The 2.8 A Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparanoid Pentasaccharide== | ==The 2.8 A Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparanoid Pentasaccharide== | ||
<StructureSection load='5uf6' size='340' side='right' caption='[[5uf6]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='5uf6' size='340' side='right'caption='[[5uf6]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5uf6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UF6 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5uf6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus Adeno-associated virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UF6 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NTO:TRISULFOAMINO+HEPARIN+PENTASACCHARIDE'>NTO</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NTO:TRISULFOAMINO+HEPARIN+PENTASACCHARIDE'>NTO</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAPSID ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272636 Adeno-associated virus])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uf6 OCA], [http://pdbe.org/5uf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uf6 RCSB], [http://www.ebi.ac.uk/pdbsum/5uf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uf6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uf6 OCA], [http://pdbe.org/5uf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uf6 RCSB], [http://www.ebi.ac.uk/pdbsum/5uf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uf6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Adeno-associated virus]] | |||
[[Category: Large Structures]] | |||
[[Category: Chapman, M]] | [[Category: Chapman, M]] | ||
[[Category: Davulcu, O]] | [[Category: Davulcu, O]] | ||
Revision as of 12:15, 1 January 2020
The 2.8 A Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparanoid PentasaccharideThe 2.8 A Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparanoid Pentasaccharide
Structural highlights
Publication Abstract from PubMedAtomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with fondaparinux, have been determined by cryoelectron microscopy at 3 A resolution. The gene therapy vector, AAV-DJ, is a hybrid of natural serotypes that was previously derived by directed evolution, selecting for hepatocyte entry and resistance to neutralization by human serum. The structure of AAV-DJ differs from that of parental serotypes in two regions where neutralizing antibodies bind, so immune escape appears to have been the primary driver of AAV-DJ's directed evolution. Fondaparinux is an analog of cell surface heparan sulfate to which several AAVs bind during entry. Fondaparinux interacts with viral arginines at a known heparin binding site, without the large conformational changes whose presence was controversial in low-resolution imaging of AAV2-heparin complexes. The glycan density suggests multi-modal binding that could accommodate sequence variation and multivalent binding along a glycan polymer, consistent with a role in attachment, prior to more specific interactions with a receptor protein mediating entry. The 2.8 A Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparinoid Pentasaccharide.,Xie Q, Spear JM, Noble AJ, Sousa DR, Meyer NL, Davulcu O, Zhang F, Linhardt RJ, Stagg SM, Chapman MS Mol Ther Methods Clin Dev. 2017 Mar 8;5:1-12. doi: 10.1016/j.omtm.2017.02.004., eCollection 2017 Jun 16. PMID:28480299[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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