6pb9: Difference between revisions
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==Crystal structure of unsaturated fatty acid bound ToxT K231A from Vibrio cholerae strain SCE256== | |||
<StructureSection load='6pb9' size='340' side='right'caption='[[6pb9]], [[Resolution|resolution]] 2.11Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6pb9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PB9 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAM:PALMITOLEIC+ACID'>PAM</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6p7r|6p7r]], [[6p7t|6p7t]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pb9 OCA], [http://pdbe.org/6pb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pb9 RCSB], [http://www.ebi.ac.uk/pdbsum/6pb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pb9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The AraC/XylS-family transcriptional regulator ToxT is the master virulence activator of Vibrio cholerae, the gram-negative bacterial pathogen that causes the diarrheal disease cholera. Unsaturated fatty acids (UFAs) found in bile inhibit the activity of ToxT. Crystal structures of inhibited ToxT bound to UFA or synthetic inhibitors have been reported, but no structure of ToxT in an active conformation had been determined. Here we present the 2.5 A structure of ToxT without an inhibitor. The structure suggests release of UFA or inhibitor leads to an increase in flexibility, allowing ToxT to adopt an active conformation that is able to dimerize and bind DNA. Small-angle X-ray scattering was used to validate a structural model of an open ToxT dimer bound to the cholera toxin promoter. The results presented here provide a detailed structural mechanism for virulence gene regulation in V. cholerae by the UFA components of bile and other synthetic ToxT inhibitors. | |||
Structural basis for virulence regulation in Vibrio cholerae by unsaturated fatty acid components of bile.,Cruite JT, Kovacikova G, Clark KA, Woodbrey AK, Skorupski K, Kull FJ Commun Biol. 2019 Nov 28;2:440. doi: 10.1038/s42003-019-0686-x. eCollection 2019. PMID:31815195<ref>PMID:31815195</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Kull, F | <div class="pdbe-citations 6pb9" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Cruite, J T]] | |||
[[Category: Kull, F J]] | |||
[[Category: Arac]] | |||
[[Category: Cholerae]] | |||
[[Category: Dna binding protein]] | |||
[[Category: Virulence regulation]] | |||
[[Category: Xyl]] | |||
Revision as of 11:20, 1 January 2020
Crystal structure of unsaturated fatty acid bound ToxT K231A from Vibrio cholerae strain SCE256Crystal structure of unsaturated fatty acid bound ToxT K231A from Vibrio cholerae strain SCE256
Structural highlights
Publication Abstract from PubMedThe AraC/XylS-family transcriptional regulator ToxT is the master virulence activator of Vibrio cholerae, the gram-negative bacterial pathogen that causes the diarrheal disease cholera. Unsaturated fatty acids (UFAs) found in bile inhibit the activity of ToxT. Crystal structures of inhibited ToxT bound to UFA or synthetic inhibitors have been reported, but no structure of ToxT in an active conformation had been determined. Here we present the 2.5 A structure of ToxT without an inhibitor. The structure suggests release of UFA or inhibitor leads to an increase in flexibility, allowing ToxT to adopt an active conformation that is able to dimerize and bind DNA. Small-angle X-ray scattering was used to validate a structural model of an open ToxT dimer bound to the cholera toxin promoter. The results presented here provide a detailed structural mechanism for virulence gene regulation in V. cholerae by the UFA components of bile and other synthetic ToxT inhibitors. Structural basis for virulence regulation in Vibrio cholerae by unsaturated fatty acid components of bile.,Cruite JT, Kovacikova G, Clark KA, Woodbrey AK, Skorupski K, Kull FJ Commun Biol. 2019 Nov 28;2:440. doi: 10.1038/s42003-019-0686-x. eCollection 2019. PMID:31815195[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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