6u3z: Difference between revisions
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<StructureSection load='6u3z' size='340' side='right'caption='[[6u3z]], [[Resolution|resolution]] 1.99Å' scene=''> | <StructureSection load='6u3z' size='340' side='right'caption='[[6u3z]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6u3z]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U3Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U3Z FirstGlance]. <br> | <table><tr><td colspan='2'>[[6u3z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Macmu Macmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U3Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U3Z FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u3z OCA], [http://pdbe.org/6u3z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u3z RCSB], [http://www.ebi.ac.uk/pdbsum/6u3z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u3z ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u3z OCA], [http://pdbe.org/6u3z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u3z RCSB], [http://www.ebi.ac.uk/pdbsum/6u3z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u3z ProSAT]</span></td></tr> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Macmu]] | |||
[[Category: Dionne, G]] | [[Category: Dionne, G]] | ||
[[Category: Shapiro, L]] | [[Category: Shapiro, L]] | ||
Revision as of 16:17, 18 December 2019
Structure of VD20_5A4 FabStructure of VD20_5A4 Fab
Structural highlights
Publication Abstract from PubMedWell-ordered HIV-1 envelope glycoprotein (Env) trimers are prioritized for clinical evaluation, and there is a need for an improved understanding about how elicited B cell responses evolve following immunization. To accomplish this, we prime-boosted rhesus macaques with clade C NFL trimers and identified 180 unique Ab lineages from approximately 1,000 single-sorted Env-specific memory B cells. We traced all lineages in high-throughput heavy chain (HC) repertoire (Rep-seq) data generated from multiple immune compartments and time points and expressed several as monoclonal Abs (mAbs). Our results revealed broad dissemination and high levels of somatic hypermutation (SHM) of most lineages, including tier 2 virus neutralizing lineages, following boosting. SHM was highest in the Ab complementarity determining regions (CDRs) but also surprisingly high in the framework regions (FRs), especially FR3. Our results demonstrate the capacity of the immune system to affinity-mature large numbers of Env-specific B cell lineages simultaneously, supporting the use of regimens consisting of repeated boosts to improve each Ab, even those belonging to less expanded lineages. Extensive dissemination and intraclonal maturation of HIV Env vaccine-induced B cell responses.,Phad GE, Pushparaj P, Tran K, Dubrovskaya V, Adori M, Martinez-Murillo P, Vazquez Bernat N, Singh S, Dionne G, O'Dell S, Bhullar K, Narang S, Sorini C, Villablanca EJ, Sundling C, Murrell B, Mascola JR, Shapiro L, Pancera M, Martin M, Corcoran M, Wyatt RT, Karlsson Hedestam GB J Exp Med. 2019 Nov 8. pii: jem.20191155. doi: 10.1084/jem.20191155. PMID:31704807[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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