2j4r: Difference between revisions
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==Structural Study of the Aquifex aeolicus PPX-GPPA enzyme== | ==Structural Study of the Aquifex aeolicus PPX-GPPA enzyme== | ||
<StructureSection load='2j4r' size='340' side='right' caption='[[2j4r]], [[Resolution|resolution]] 2.71Å' scene=''> | <StructureSection load='2j4r' size='340' side='right'caption='[[2j4r]], [[Resolution|resolution]] 2.71Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2j4r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J4R FirstGlance]. <br> | <table><tr><td colspan='2'>[[2j4r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J4R FirstGlance]. <br> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Aquifex aeolicus huber and stetter 2001]] | [[Category: Aquifex aeolicus huber and stetter 2001]] | ||
[[Category: Large Structures]] | |||
[[Category: Kristensen, O]] | [[Category: Kristensen, O]] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
Revision as of 22:05, 11 December 2019
Structural Study of the Aquifex aeolicus PPX-GPPA enzymeStructural Study of the Aquifex aeolicus PPX-GPPA enzyme
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe crystal structure of the prototype exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family member from Aquifex aeolicus in complex with the intracellular second messenger guanosine tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is identified as E119. The dual specificity established for the Escherichia coli homolog is shown to be compatible with a common active site for guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and different degrees of closure between the two domains of the enzyme are associated with substrate binding. The arginines R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation. Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp.,Kristensen O, Ross B, Gajhede M J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:18155044[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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