5hyo: Difference between revisions
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==X-Ray Structure of Unbound Porcine Epidemic Diarrhea Virus 3CLpro== | ==X-Ray Structure of Unbound Porcine Epidemic Diarrhea Virus 3CLpro== | ||
<StructureSection load='5hyo' size='340' side='right' caption='[[5hyo]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='5hyo' size='340' side='right'caption='[[5hyo]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5hyo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pedv Pedv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HYO FirstGlance]. <br> | <table><tr><td colspan='2'>[[5hyo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pedv Pedv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HYO FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5hyo" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5hyo" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Virus protease|Virus protease]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Pedv]] | [[Category: Pedv]] | ||
[[Category: John, S E.St]] | [[Category: John, S E.St]] | ||
Revision as of 19:14, 11 December 2019
X-Ray Structure of Unbound Porcine Epidemic Diarrhea Virus 3CLproX-Ray Structure of Unbound Porcine Epidemic Diarrhea Virus 3CLpro
Structural highlights
Publication Abstract from PubMedPorcine epidemic diarrhea virus (PEDV) is a coronavirus that infects pigs and can have mortality rates approaching 100% in piglets, causing serious economic impact. The 3C-like protease (3CL(pro)) is essential for the coronaviral life cycle and is an appealing target for the development of therapeutics. We report the expression, purification, crystallization and 2.10 A X-ray structure of 3CL(pro) from PEDV. Analysis of the PEDV 3CL(pro) structure and comparison to other coronaviral 3CL(pro)'s from the same alpha-coronavirus phylogeny shows that the overall structures and active site architectures across 3CL(pro)'s are conserved, with the exception of a loop that comprises the protease S2 pocket. We found a known inhibitor of severe acute respiratory syndrome coronavirus (SARS-CoV) 3CL(pro), (R)-16, to have inhibitor activity against PEDV 3CL(pro), despite that SARS-3CL(pro) and PEDV 3CL(pro) share only 45.4% sequence identity. Structural comparison reveals that the majority of residues involved in (R)-16 binding to SARS-3CL(pro) are conserved in PEDV-3CL(pro); however, the sequence variation and positional difference in the loop forming the S2 pocket may account for large observed difference in IC50 values. This work advances our understanding of the subtle, but important, differences in coronaviral 3CL(pro) architecture and contributes to the broader structural knowledge of coronaviral 3CL(pro)'s. X-Ray Structure and Inhibition of 3C-like Protease from Porcine Epidemic Diarrhea Virus.,St John SE, Anson BJ, Mesecar AD Sci Rep. 2016 May 13;6:25961. doi: 10.1038/srep25961. PMID:27173881[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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