Pseudouridine synthase: Difference between revisions
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<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'> | <StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'> | ||
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==Function== | |||
[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction: | [[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction: | ||
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== Structural highlights == | == Structural highlights == | ||
The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]). | The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]). | ||
== 3D Structures of pseudouridine synthase == | |||
[[Pseudouridine synthase 3D structures]] | |||
</StructureSection> | </StructureSection> | ||
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* Pseudouridine synthase | * Pseudouridine synthase | ||
**[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human<br /> | |||
**[[4nz7]] - hPUS Pus1 catalytic domain (mutant)<br /> | |||
**[[1ze1]] – TmPUS B – ''Thermotoga maritima''<br /> | |||
**[[1vio]] - HiPUS RsuA – ''Haemophilus influenzae''<br /> | |||
**[[6gut]] – HiPUS/PILA<br /> | |||
**[[1dj0]] – EcPUS I – ''Escherichia coli''<br /> | **[[1dj0]] – EcPUS I – ''Escherichia coli''<br /> | ||
**[[ | **[[1ksk]], [[1ksl]], [[1ksv]] – EcPUS RsuA <br /> | ||
**[[4lab]] - EcPUS RluB catalytic domain<br /> | |||
**[[ | |||
**[[1xpi]] - EcPUS RluC catalytic domain<br /> | **[[1xpi]] - EcPUS RluC catalytic domain<br /> | ||
**[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <br /> | **[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <br /> | ||
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**[[2oml]] - EcPUS RluE catalytic domain<br /> | **[[2oml]] - EcPUS RluE catalytic domain<br /> | ||
**[[2gml]] - EcPUS RluF catalytic domain<br /> | **[[2gml]] - EcPUS RluF catalytic domain<br /> | ||
**[[1vs3]] – PUS TruA – ''Thermus thermophilus''<br /> | **[[1vs3]] – PUS TruA – ''Thermus thermophilus''<br /> | ||
**[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<br /> | **[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<br /> | ||
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**[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<br /> | **[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<br /> | ||
**[[2i82]] – EcPUS RluA + RNA<br /> | **[[2i82]] – EcPUS RluA + RNA<br /> | ||
**[[4lgt]] – EcPUS RluB + RNA<br /> | |||
**[[3dh3]] - EcPUS RluF + RNA<br /> | **[[3dh3]] - EcPUS RluF + RNA<br /> | ||
**[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<br /> | **[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<br /> | ||
**[[1r3e]], [[1r3f]] - EcPUS B + RNA<br /> | |||
**[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<br /> | **[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<br /> | ||
**[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<br /> | **[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<br /> | ||
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**[[1ze2]] - TmPUS B + RNA<br /> | **[[1ze2]] - TmPUS B + RNA<br /> | ||
**[[2ab4]] - TmPUS B (mutant) + RNA<br /> | **[[2ab4]] - TmPUS B (mutant) + RNA<br /> | ||
* Probable pseudouridine synthase | * Probable pseudouridine synthase | ||