6b0b: Difference between revisions
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==Crystal structure of human APOBEC3H== | ==Crystal structure of human APOBEC3H== | ||
<StructureSection load='6b0b' size='340' side='right' caption='[[6b0b]], [[Resolution|resolution]] 3.28Å' scene=''> | <StructureSection load='6b0b' size='340' side='right'caption='[[6b0b]], [[Resolution|resolution]] 3.28Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6b0b]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dissp Dissp], [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B0B FirstGlance]. <br> | <table><tr><td colspan='2'>[[6b0b]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dissp Dissp], [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B0B FirstGlance]. <br> | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Human]] | [[Category: Human]] | ||
[[Category: Large Structures]] | |||
[[Category: Aihara, H]] | [[Category: Aihara, H]] | ||
[[Category: Banerjee, S]] | [[Category: Banerjee, S]] | ||
Revision as of 12:48, 4 December 2019
Crystal structure of human APOBEC3HCrystal structure of human APOBEC3H
Structural highlights
Publication Abstract from PubMedHuman APOBEC3H and homologous single-stranded DNA cytosine deaminases are unique to mammals. These DNA-editing enzymes function in innate immunity by restricting the replication of viruses and transposons. APOBEC3H also contributes to cancer mutagenesis. Here, we address the fundamental nature of RNA in regulating human APOBEC3H activities. APOBEC3H co-purifies with RNA as an inactive protein, and RNase A treatment enables strong DNA deaminase activity. RNA-binding-defective mutants demonstrate clear separation of function by becoming DNA hypermutators. Biochemical and crystallographic data demonstrate a mechanism in which double-stranded RNA mediates enzyme dimerization. Additionally, APOBEC3H separation-of-function mutants show that RNA binding is required for cytoplasmic localization, packaging into HIV-1 particles, and antiviral activity. Overall, these results support a model in which structured RNA negatively regulates the potentially harmful DNA deamination activity of APOBEC3H while, at the same time, positively regulating its antiviral activity. The Antiviral and Cancer Genomic DNA Deaminase APOBEC3H Is Regulated by an RNA-Mediated Dimerization Mechanism.,Shaban NM, Shi K, Lauer KV, Carpenter MA, Richards CM, Salamango D, Wang J, Lopresti MW, Banerjee S, Levin-Klein R, Brown WL, Aihara H, Harris RS Mol Cell. 2018 Jan 4;69(1):75-86.e9. doi: 10.1016/j.molcel.2017.12.010. Epub 2017, Dec 28. PMID:29290613[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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