Proteinase: Difference between revisions

[C] The molecular models of the IRD bound HaTry predicted several atomic interactions with a reactive loop of inhibitors that also explained the contribution of the solvent exposed reactive loop. There are several hydrogen bonds in the <scene name='Journal:JBSD:39/Ird9/3'>IRD-9-HaTry complex</scene>. ARG-39 from <scene name='Journal:JBSD:39/Cv/17'>IRD-12</scene> reactive site formed two hydrogen bonds with the residues of the HaTry active site. In <scene name='Journal:JBSD:39/Ird9/2'>case of IRD-7</scene>, side chain of LYS-39 residue of reactive loop form one hydrogen bond each, with carboxyl oxygen atom of HIS-50. MD simulations provides structural insight into an importance of inter/intra molecular hydrogen bonds and its effect on the interaction between protease and PIs. The results of this analysis were corroborated with previous reports. Post simulation analysis also explained experimentally observed increase in binding affinity, hence activity of IRD-9 towards proteases. See also <ref name="Barrette-Ng">PMID: 12684499</ref> <ref name="Dunse">PMID: 20696921</ref> <ref name="Tamhane">PMID: 19393726</ref> <ref name="Tamhane1">PMID: 15715970</ref>.

</StructureSection>

*'''Proteinase A or saccharopepsin'''

**[[2sga]] – SgPRO – ''Streptomyces griseus''<br />

**[[1sgc]] - SgPRO + chymostatin A<br />

**[[3sga]], [[4sga]], [[5sga]] - SgPRO + polypeptide inhibitor<br />

**[[4fvd]] – hevPRO 2A + peptide – human enterovirus <br />

**[[4fvb]] – hevPRO 2A (mutant) <br />

*'''Aspartic Proteinase'''  

**[[2asi]] – PRO – ''Rhizomucor miehei''<br />

**[[1zap]] – CaPRO – ''Candida albicans''<br />

**[[1izd]] - AoPRO – ''Aspergillus oryzae''<br />

**[[1j71]] - PRO + polypeptide inhibitor – ''Candida tropicalis''<br />

**[[1wkr]] - PRO + polypeptide-statin inhibitor – ''Irpex lacteus''

*'''Cysteine Proteinase''' or '''gingipain'''

**[[2hrv]] – PRO 2A – human rhinovirus<br />

**[[3m1h]], [[4itc]] – PgPRO adhesion domain residues 982-1154 – ''Porphyromonas gingivalis'' <br />

**[[5mun]] – PgPRO residues 20-228 <br />

**[[2fo5]] – PRO residues 133-356 + leupeptin – ''Hordenum vulgare'' <br />

**[[1x9y]] – SaPRO – ''Staphylococcus aureus'' <br />

**[[1y4h]], [[1pxv]] – SaPRO + PRO inhibitor <br />

*'''Serine Proteinase'''

**[[3s9a]], [[3s9b]] – RvPRO – Siamese Russell’s viper<br />

**[[1po0]], [[1op2]] – PRO – Chinese moccasin <br />

**[[1qy8]] – SaPRO <br />

**[[1mbm]] – PRO – equine arteritis virus <br />

**[[1ga1]], [[1ga4]], [[1ga6]], [[1nlu]] – PsPRO + iodotyrostatin fragment – ''Pseudomonas''<br />

**[[6m8w]], [[6m8y]], [[6m8f]], [[6m9c]], [[6m9d]] - PsPRO + polypeptide inhibitor<br />

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