5kkd: Difference between revisions
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==Truncated hemolysin A Y134A from P. mirabilis at 2.1 Angstroms resolution crystallized in a high salt condition== | ==Truncated hemolysin A Y134A from P. mirabilis at 2.1 Angstroms resolution crystallized in a high salt condition== | ||
<StructureSection load='5kkd' size='340' side='right' caption='[[5kkd]], [[Resolution|resolution]] 2.13Å' scene=''> | <StructureSection load='5kkd' size='340' side='right'caption='[[5kkd]], [[Resolution|resolution]] 2.13Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5kkd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29906 Atcc 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KKD FirstGlance]. <br> | <table><tr><td colspan='2'>[[5kkd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29906 Atcc 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KKD FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5kkd" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5kkd" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Hemolysin 3D structures|Hemolysin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Atcc 29906]] | [[Category: Atcc 29906]] | ||
[[Category: Large Structures]] | |||
[[Category: Bhattacharyya, B]] | [[Category: Bhattacharyya, B]] | ||
[[Category: Novak, W R.P]] | [[Category: Novak, W R.P]] | ||
Revision as of 10:45, 27 November 2019
Truncated hemolysin A Y134A from P. mirabilis at 2.1 Angstroms resolution crystallized in a high salt conditionTruncated hemolysin A Y134A from P. mirabilis at 2.1 Angstroms resolution crystallized in a high salt condition
Structural highlights
Publication Abstract from PubMedWild-type and variant forms of HpmA265 (truncated hemolysin A) from Proteus mirabilis reveal a right-handed, parallel beta-helix capped and flanked by segments of antiparallel beta-strands. The low-salt crystal structures form a dimeric structure via the implementation of on-edge main-chain hydrogen bonds donated by residues 243-263 of adjacent monomers. Surprisingly, in the high-salt structures of two variants, Y134A and Q125A-Y134A, a new dimeric interface is formed via main-chain hydrogen bonds donated by residues 203-215 of adjacent monomers, and a previously unobserved tetramer is formed. In addition, an eight-stranded antiparallel beta-sheet is formed from the flap regions of crystallographically related monomers in the high-salt structures. This new interface is possible owing to additional proteolysis of these variants after Tyr240. The interface formed in the high-salt crystal forms of hemolysin A variants may mimic the on-edge beta-strand positioning used in template-assisted hemolytic activity. Proteolysis of truncated hemolysin A yields a stable dimerization interface.,Novak WR, Bhattacharyya B, Grilley DP, Weaver TM Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):138-145. doi:, 10.1107/S2053230X17002102. Epub 2017 Feb 21. PMID:28291749[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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