5hhf: Difference between revisions
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==Crystal structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant H63A with covalent FAD-Galactopyranose and bound UDP== | ==Crystal structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant H63A with covalent FAD-Galactopyranose and bound UDP== | ||
<StructureSection load='5hhf' size='340' side='right' caption='[[5hhf]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='5hhf' size='340' side='right'caption='[[5hhf]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5hhf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HHF FirstGlance]. <br> | <table><tr><td colspan='2'>[[5hhf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HHF FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5hhf" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5hhf" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[UDP-galactopyranose mutase|UDP-galactopyranose mutase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Aspfm]] | [[Category: Aspfm]] | ||
[[Category: Large Structures]] | |||
[[Category: UDP-galactopyranose mutase]] | [[Category: UDP-galactopyranose mutase]] | ||
[[Category: Tanner, J J]] | [[Category: Tanner, J J]] | ||
Revision as of 10:35, 27 November 2019
Crystal structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant H63A with covalent FAD-Galactopyranose and bound UDPCrystal structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant H63A with covalent FAD-Galactopyranose and bound UDP
Structural highlights
Publication Abstract from PubMedUDP-galactopyranose mutase (UGM) plays an essential role in galactofuranose biosynthesis in pathogens by catalyzing the conversion of UDP-galactopyranose to UDP-galactofuranose. Here we report the first crystal structure of a covalent intermediate in the UGM reaction. The 2.3 A resolution structure reveals UDP bound in the active site and galactopyranose linked to the FAD through a covalent bond between the anomeric C of galactopyranose and N5 of the FAD. The structure confirms the role of the flavin as nucleophile and supports the hypothesis that the proton destined for O5 of galactofuranose is shuttled from N5 of the FAD via O4 of the FAD. In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction.,Mehra-Chaudhary R, Dai Y, Sobrado P, Tanner JJ Biochemistry. 2016 Feb 16;55(6):833-6. doi: 10.1021/acs.biochem.6b00035. Epub, 2016 Feb 4. PMID:26836146[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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