154d: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:154d.jpg|left|200px]] | [[Image:154d.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_154d", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_154d| PDB=154d | SCENE= }} | |||
}} | |||
'''DNA DISTORTION IN BIS-INTERCALATED COMPLEXES''' | '''DNA DISTORTION IN BIS-INTERCALATED COMPLEXES''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=154D OCA]. | |||
==Reference== | ==Reference== | ||
DNA distortion in bis-intercalated complexes., Peek ME, Lipscomb LA, Bertrand JA, Gao Q, Roques BP, Garbay-Jaureguiberry C, Williams LD, Biochemistry. 1994 Apr 5;33(13):3794-800. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8142381 8142381] | DNA distortion in bis-intercalated complexes., Peek ME, Lipscomb LA, Bertrand JA, Gao Q, Roques BP, Garbay-Jaureguiberry C, Williams LD, Biochemistry. 1994 Apr 5;33(13):3794-800. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8142381 8142381] | ||
[[Category: Bertrand, J A.]] | [[Category: Bertrand, J A.]] | ||
[[Category: Gao, Q.]] | [[Category: Gao, Q.]] | ||
| Line 31: | Line 27: | ||
[[Category: Roques, B P.]] | [[Category: Roques, B P.]] | ||
[[Category: Williams, L D.]] | [[Category: Williams, L D.]] | ||
[[Category: | [[Category: Complexed with drug]] | ||
[[Category: | [[Category: Double helix]] | ||
[[Category: | [[Category: Modified]] | ||
[[Category: | [[Category: Right handed dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:31:07 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 09:31, 2 May 2008
DNA DISTORTION IN BIS-INTERCALATED COMPLEXES
OverviewOverview
The bis-intercalators Flexi-Di and ditercalinium are synthetic dimers that bis-intercalate into DNA and cause cell death in prokaryotes from futile and abortive repair of DNA. Each is composed of two 7H-pyridocarbazole units and a linker. Flexi-Di has a flexible spermine-like linker while ditercalinium has a rigid bis(ethylpiperidinium) linker. This report, describing the 2.5-A X-ray structure of Flexi-Di complexed with [d(BrCGCG)]2, appears to be the first report of a three-dimensional structure of a DNA complex with a bis-intercalator with a flexible linker. DNA complex formation with a ditercalinium analog having a flexible linker was not anticipated to yield unstacked and bent DNA as was observed in the previously reported ditercalinium.[d(CGCG)]2 complex. Surprisingly, the DNA in the Flexi-Di complex is bent to a degree exceeding that of the ditercalinium complex. A comparison of the DNA complexes of Flexi-Di and ditercalinium has allowed us to propose a mechanism by which these bis-intercalators distort DNA. We propose that this class of bis-intercalators pulls the internal base pairs into the major groove and pushes the external base pairs into the minor groove. The result is a bend toward the minor groove. It appears that hydrogen bonds between the linker and the internal guanines effectively pull the central base pairs of the complex out into the major groove. At the external regions of the complex, stacking interactions between the chromophores and terminal base pairs effectively push the terminal base pairs into the minor groove. The result of this push/pull combination is to bend the DNA.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
DNA distortion in bis-intercalated complexes., Peek ME, Lipscomb LA, Bertrand JA, Gao Q, Roques BP, Garbay-Jaureguiberry C, Williams LD, Biochemistry. 1994 Apr 5;33(13):3794-800. PMID:8142381 Page seeded by OCA on Fri May 2 09:31:07 2008