153l: Difference between revisions

Revision as of 09:31, 2 May 2008

THE REFINED STRUCTURES OF GOOSE LYSOZYME AND ITS COMPLEX WITH A BOUND TRISACCHARIDE SHOW THAT THE "GOOSE-TYPE LYSOZYMES LACK A CATALYTIC ASPARTATE

OverviewOverview

The structure of goose egg-white lysozyme (GEWL) has been refined to an R-value of 15.9% at 1.6 A resolution. Details of the structure determination, the refinement and the structure itself are presented. The structure of a complex of the enzyme with the trisaccharide of N-acetyl glucosamine has also been determined and refined at 1.6 A resolution. The trisaccharide occupies sites analogous to the B, C and D subsites of chicken (HEWL) and phage T4 (T4L) lysozymes. All three lysozymes (GEWL, HEWL and T4L) display the same characteristic set of bridging hydrogen bonds between backbone atoms of the protein and the 2-acetamido group of the saccharide in subsite C. Glu73 of GEWL is seen to correspond closely to Glu35 of HEWL (and to Glu11 of T4L) and supports the established view that this group is critically involved in the catalytic mechanism. There is, however, no obvious residue in goose lysozyme that is a counterpart of Asp52 of chicken lysozyme (or of Asp20 in T4L), suggesting that a second acidic residue is not essential for the catalytic activity of goose lysozyme, and may not be required for the activity of other lysozymes.

About this StructureAbout this Structure

153L is a Single protein structure. Full crystallographic information is available from OCA.

ReferenceReference

The refined structures of goose lysozyme and its complex with a bound trisaccharide show that the "goose-type" lysozymes lack a catalytic aspartate residue., Weaver LH, Grutter MG, Matthews BW, J Mol Biol. 1995 Jan 6;245(1):54-68. PMID:7823320 Page seeded by OCA on Fri May 2 09:31:01 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:153l.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 153l |SIZE=350|CAPTION= <scene name='initialview01'>153l</scene>, resolution 1.6&Aring;
+The line below this paragraph, containing "STRUCTURE_153l", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_153l|  PDB=153l  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=153l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=153l OCA], [http://www.ebi.ac.uk/pdbsum/153l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=153l RCSB]</span>
-}}
 '''THE REFINED STRUCTURES OF GOOSE LYSOZYME AND ITS COMPLEX WITH A BOUND TRISACCHARIDE SHOW THAT THE "GOOSE-TYPE LYSOZYMES LACK A CATALYTIC ASPARTATE'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=153L OCA].
+L is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=153L OCA].
 ==Reference==
@@ Line 28: / Line 25: @@
 [[Category: Matthews, B W.]]
 [[Category: Weaver, L H.]]
-[[Category: hydrolase(o-glycosyl)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:31:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:28:24 2008''