5t5h: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit== | ==Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit== | ||
<StructureSection load='5t5h' size='340' side='right' caption='[[5t5h]], [[Resolution|resolution]] 2.54Å' scene=''> | <StructureSection load='5t5h' size='340' side='right'caption='[[5t5h]], [[Resolution|resolution]] 2.54Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5t5h]] is a 46 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T5H FirstGlance]. <br> | <table><tr><td colspan='2'>[[5t5h]] is a 46 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T5H FirstGlance]. <br> | ||
| Line 8: | Line 8: | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5h OCA], [http://pdbe.org/5t5h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t5h RCSB], [http://www.ebi.ac.uk/pdbsum/5t5h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5h ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5h OCA], [http://pdbe.org/5t5h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t5h RCSB], [http://www.ebi.ac.uk/pdbsum/5t5h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/Q4DXW6_TRYCC Q4DXW6_TRYCC]] Binds to the 23S rRNA.[RuleBase:RU000576] | [[http://www.uniprot.org/uniprot/Q4DXW6_TRYCC Q4DXW6_TRYCC]] Binds to the 23S rRNA.[RuleBase:RU000576] | ||
| Line 24: | Line 23: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Trypanosoma cruzi]] | [[Category: Trypanosoma cruzi]] | ||
[[Category: Espina, N]] | [[Category: Espina, N]] | ||
Revision as of 19:38, 20 November 2019
Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunitStructure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit
Structural highlights
Function[Q4DXW6_TRYCC] Binds to the 23S rRNA.[RuleBase:RU000576] Publication Abstract from PubMedRibosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA expansion segments, and r-protein variations compared with other eukaryotic ribosomes. To investigate the architecture of the trypanosomatid ribosomes, we determined the 2.5-A structure of the Trypanosoma cruzi ribosome large subunit by single-particle cryo-EM. Examination of this structure and comparative analysis of the yeast ribosomal assembly pathway allowed us to develop a stepwise assembly model for the eight pieces of the large subunit rRNAs and a number of ancillary "glue" proteins. This model can be applied to the characterization of Trypanosoma brucei and Leishmania spp. ribosomes as well. Together with other details, our atomic-level structure may provide a foundation for structure-based design of antitrypanosome drugs. Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.,Liu Z, Gutierrez-Vargas C, Wei J, Grassucci RA, Ramesh M, Espina N, Sun M, Tutuncuoglu B, Madison-Antenucci S, Woolford JL Jr, Tong L, Frank J Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12174-12179. Epub 2016 Oct 10. PMID:27791004[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||