6mi6: Difference between revisions

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 ==STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH AN ADP ANALOG==
-<StructureSection load='6mi6' size='340' side='right' caption='[[6mi6]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
+<StructureSection load='6mi6' size='340' side='right'caption='[[6mi6]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6mi6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MI6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6mi6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MI6 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=JSJ:5-O-[(S)-hydroxy{[(S)-hydroxy{[(1-hydroxy-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl]disulfanyl}phosphoryl]oxy}phosphoryl]adenosine'>JSJ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cheA, TM_0702 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mi6 OCA], [http://pdbe.org/6mi6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mi6 RCSB], [http://www.ebi.ac.uk/pdbsum/6mi6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mi6 ProSAT]</span></td></tr>
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 == Function ==
 [[http://www.uniprot.org/uniprot/CHEA_THEMA CHEA_THEMA]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either CheB or CheY (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Site-directed spin labeling of proteins by chemical modification of engineered cysteine residues with the molecule MTSSL (1-Oxyl-2,2,5,5-tetramethylpyrroline-3-methyl methanethiosulfonate) has been an invaluable tool for conducting double electron electron resonance (DEER) spectroscopy experiments. However, this method is generally limited to recombinant proteins with a limited number of reactive Cys residues that when modified will not impair protein function. Here we present a method that allows for spin-labeling of protein nucleotide binding sites by adenosine diphosphate (ADP) modified with a nitroxide moiety on the beta-phosphate (ADP-beta-S-SL). The synthesis of this ADP analog is straightforward and isolation of pure product is readily achieved on a standard reverse-phase high-performance liquid chromatography (HPLC) system. Furthermore, analyses of isolated ADP-beta-S-SL by LC-mass spectrometry confirm that the molecule is extremely stable under ambient conditions. The crystal structure of ADP-beta-S-SL bound to the ATP pocket of the histidine kinase CheA reveals specific targeting of the probe, whose nitroxide moiety is mobile on the protein surface. Continuous wave and pulsed ESR measurements demonstrate the capability of ADP-beta-S-SL to report on active site environment and provide reliable DEER distance constraints.
+Nucleotide Spin Labeling for ESR Spectroscopy of ATP-Binding Proteins.,Muok AR, Chua TK, Le H, Crane BR Appl Magn Reson. 2018 Dec;49(12):1385-1395. doi: 10.1007/s00723-018-1070-6. Epub , 2018 Nov 14. PMID:30686862<ref>PMID:30686862</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 6mi6" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
+== References ==
+<references/>
 __TOC__
 </StructureSection>
 [[Category: Histidine kinase]]
+[[Category: Large Structures]]
+[[Category: Thema]]
 [[Category: Chua, T K]]
 [[Category: Crane, B R]]