1fw6

CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEXCRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX

Structural highlights 1fw6 is a 4 chain structure with sequence from Atcc 25104. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , , NonStd Res: Related: 1ewq, 1ewr Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [MUTS_THEAQ] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpaired bases embedded in duplex DNA and activating endo- and exonucleases to remove the mismatch. Members of the MutS family also possess a conserved ATPase activity that belongs to the ATP binding cassette (ABC) superfamily. Here we report the crystal structure of a ternary complex of MutS-DNA-ADP and assays of initiation of mismatch repair in conjunction with perturbation of the composite ATPase active site by mutagenesis. These studies indicate that MutS has to bind both ATP and the mismatch DNA simultaneously in order to activate the other mismatch repair proteins. We propose that the MutS ATPase activity plays a proofreading role in DNA mismatch repair, verification of mismatch recognition, and authorization of repair. Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair.,Junop MS, Obmolova G, Rausch K, Hsieh P, Yang W Mol Cell. 2001 Jan;7(1):1-12. PMID:11172706 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 1fw6, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image