2pjr: Difference between revisions

Revision as of 13:57, 30 April 2008

HELICASE PRODUCT COMPLEX

OverviewOverview

We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of different steps on the catalytic pathway. One of the structures is of a complex with a nonhydrolyzable analog of ATP and is thus a "substrate" complex. The other structure contains a bound sulphate ion that sits in a position equivalent to that occupied by the phosphate ion produced after ATP hydrolysis, thereby mimicking a "product" complex. In both complexes, the protein is monomeric. Large and distinct conformational changes occur on binding DNA and the nucleotide cofactor. Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism.

About this StructureAbout this Structure

2PJR is a Protein complex structure of sequences from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism., Velankar SS, Soultanas P, Dillingham MS, Subramanya HS, Wigley DB, Cell. 1999 Apr 2;97(1):75-84. PMID:10199404 Page seeded by OCA on Wed Apr 30 13:57:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2pjr.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2pjr |SIZE=350|CAPTION= <scene name='initialview01'>2pjr</scene>, resolution 2.9&Aring;
+The line below this paragraph, containing "STRUCTURE_2pjr", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2pjr|  PDB=2pjr  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjr OCA], [http://www.ebi.ac.uk/pdbsum/2pjr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pjr RCSB]</span>
-}}
 '''HELICASE PRODUCT COMPLEX'''
@@ Line 30: / Line 27: @@
 [[Category: Velankar, S S.]]
 [[Category: Wigley, D B.]]
-[[Category: dna]]
+[[Category: Dna]]
-[[Category: helicase pcra]]
+[[Category: Helicase pcra]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: product complex]]
+[[Category: Product complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:57:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:36:52 2008''