2ok0: Difference between revisions

Revision as of 13:57, 30 April 2008

Fab ED10-DNA complex

OverviewOverview

DNA recognition by antibodies is a key feature of autoimmune diseases, yet model systems with structural information are very limited. The monoclonal antibody ED-10 recognizes one of the strands of the DNA duplex used in the immunogenic complex. Modifications of the 5' end decrease the binding affinity and short oligonucleotides retain high binding affinity. We determined crystal structures for the Fab bound to a 6-mer oligonucleotide containing the specific sequence that raised the antibody and compared it with the unliganded Fab. Only the first two bases from the 5' end (dTdC) display electron density and we observe four key hydrogen bonds at the interface. The thymine ring is stacked between TrpH50 and TrpH95, and the cytosine ring is packed against TyrL32. Upon DNA binding, TyrH97 and TrpH95 rearrange to allow subnanomolar binding affinity, five orders of magnitude higher than other reported complexes, possibly because of having gone through affinity maturation. This structure represents the first bona fide antibody DNA immunogen complex described in atomic detail.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Specific recognition of a DNA immunogen by its elicited antibody., Sanguineti S, Centeno Crowley JM, Lodeiro Merlo MF, Cerutti ML, Wilson IA, Goldbaum FA, Stanfield RL, de Prat-Gay G, J Mol Biol. 2007 Jun 29;370(1):183-95. Epub 2007 Apr 24. PMID:17512945 Page seeded by OCA on Wed Apr 30 13:57:30 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2ok0.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2ok0", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|RELATEDENTRY=[[2ojz|2OJZ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ok0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ok0 OCA], [http://www.ebi.ac.uk/pdbsum/2ok0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ok0 RCSB]</span>
-}}
 '''Fab ED10-DNA complex'''
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 ==About this Structure==
-OK0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OK0 OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OK0 OCA].
 ==Reference==
 Specific recognition of a DNA immunogen by its elicited antibody., Sanguineti S, Centeno Crowley JM, Lodeiro Merlo MF, Cerutti ML, Wilson IA, Goldbaum FA, Stanfield RL, de Prat-Gay G, J Mol Biol. 2007 Jun 29;370(1):183-95. Epub 2007 Apr 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17512945 17512945]
-[[Category: Mus musculus]]
-[[Category: Protein complex]]
 [[Category: Prat-Gay, G de.]]
 [[Category: Sanguineti, S.]]
 [[Category: Stanfield, R L.]]
 [[Category: Wilson, I A.]]
-[[Category: immunoglobulin fold]]
+[[Category: Immunoglobulin fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:57:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:18:53 2008''