5uz9: Difference between revisions

==Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex==

== Structural highlights ==

<table><tr><td colspan='2'>[[5uz9]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpd31 Bpd31], [http://en.wikipedia.org/wiki/Pseab Pseab] and [http://en.wikipedia.org/wiki/Pseudomonas_phage_jbd30 Pseudomonas phage jbd30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UZ9 FirstGlance]. <br>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uz9 OCA], [http://pdbe.org/5uz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uz9 RCSB], [http://www.ebi.ac.uk/pdbsum/5uz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uz9 ProSAT]</span></td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/CAS6_PSEAB CAS6_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:20829488</ref> <ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/CSY3_PSEAB CSY3_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/CSY2_PSEAB CSY2_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Absolutely required for crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/ACR30_BPD31 ACR30_BPD31]] Allows the phage to evade the CRISPR/Cas system type I-F.<ref>PMID:23242138</ref>   

==See Also==

== References ==

<references/>

</StructureSection>

[[Category: Bpd31]]

[[Category: Pseab]]

[[Category: Pseudomonas phage jbd30]]