1zo3: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==The P-site and P/E-site tRNA structures fitted to P/I site codon.== | ==The P-site and P/E-site tRNA structures fitted to P/I site codon.== | ||
<StructureSection load='1zo3' size='340' side='right' caption='[[1zo3]], [[Resolution|resolution]] 13.80Å' scene=''> | <StructureSection load='1zo3' size='340' side='right'caption='[[1zo3]], [[Resolution|resolution]] 13.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1zo3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZO3 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1zo3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZO3 FirstGlance]. <br> | ||
| Line 16: | Line 16: | ||
</div> | </div> | ||
<div class="pdbe-citations 1zo3" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1zo3" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 21: | Line 24: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | |||
[[Category: Allen, G S]] | [[Category: Allen, G S]] | ||
[[Category: Ehrenberg, M]] | [[Category: Ehrenberg, M]] | ||
Revision as of 10:05, 6 November 2019
The P-site and P/E-site tRNA structures fitted to P/I site codon.The P-site and P/E-site tRNA structures fitted to P/I site codon.
Structural highlights
Publication Abstract from PubMedThe 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex. The cryo-EM structure of a translation initiation complex from Escherichia coli.,Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J Cell. 2005 Jun 3;121(5):703-12. PMID:15935757[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||