Malate dehydrogenase: Difference between revisions
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==Evolutionary Divergence== | ==Evolutionary Divergence== | ||
The evolutionary past of MDH shows a divergence to form lactate dehydrogenase (LDH) which functions in a very similar way to MDH. Although there is a very low sequence conservation among MDH and LDH [http://blast.ncbi.nlm.nih.gov/Blast.cgi] the structure of the enzyme has remained relatively conserved. The key difference between the two is in the substrate: LDH catalyzes pyruvate to lactate. | The evolutionary past of MDH shows a divergence to form lactate dehydrogenase (LDH) which functions in a very similar way to MDH. Although there is a very low sequence conservation among MDH and LDH [http://blast.ncbi.nlm.nih.gov/Blast.cgi] the structure of the enzyme has remained relatively conserved. The key difference between the two is in the substrate: LDH catalyzes pyruvate to lactate. | ||
== 3D Structures of Malate Dehydrogenase == | |||
[[Malate Dehydrogenase 3D structures]] | |||
</StructureSection> | </StructureSection> | ||
== 3D Structures of Malate Dehydrogenase == | == 3D Structures of Malate Dehydrogenase == | ||
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* Holo-MDH | * Holo-MDH | ||
**[[2dfd]], [[4wlv]] – hMDH2 +NAD – human<br /> | |||
**[[4mdh]] – pMDH+NAD - pig<br /> | |||
**[[2x0r]] – HmMDH (mutant)+NAD - ''Haloarcula marismortui''<br /> | **[[2x0r]] – HmMDH (mutant)+NAD - ''Haloarcula marismortui''<br /> | ||
**[[1o6z]] - HmMDH (mutant)+NADH<br /> | **[[1o6z]] - HmMDH (mutant)+NADH<br /> | ||
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**[[2x0j]] - AfMDH+etheno-NAD<br /> | **[[2x0j]] - AfMDH+etheno-NAD<br /> | ||
**[[1ib6]], [[1ie3]] – EcMDH (mutant)+NAD - ''Escherichia coli''<br /> | **[[1ib6]], [[1ie3]] – EcMDH (mutant)+NAD - ''Escherichia coli''<br /> | ||
**[[3i0p]] – MDH+NAD – ''Entamoeba histolytica''<br /> | **[[3i0p]] – MDH+NAD – ''Entamoeba histolytica''<br /> | ||
**[[3gvh]] – BmMDH+NAD – ''Brucella melitensis''<br /> | **[[3gvh]] – BmMDH+NAD – ''Brucella melitensis''<br /> | ||
**[[1wze]] – TfMDH (mutant)+NAD – ''Thermus flavus''<br /> | **[[1wze]] – TfMDH (mutant)+NAD – ''Thermus flavus''<br /> | ||
**[[1wzi]] - TfMDH (mutant)+NDP<br /> | **[[1wzi]] - TfMDH (mutant)+NDP<br /> | ||
**[[1bmd]] – TfMDH+NAD<br /> | **[[1bmd]] – TfMDH+NAD<br /> | ||
**[[1y7t]] – TtMDH+NADPH – ''Thermus thermophilus''<br /> | **[[1y7t]] – TtMDH+NADPH – ''Thermus thermophilus''<br /> | ||
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**[[1civ]] – MDH+NADP – ''Flaveria bidentis''<br /> | **[[1civ]] – MDH+NADP – ''Flaveria bidentis''<br /> | ||
**[[1b8u]], [[1b8v]] – AaMDH+NAD - ''Aquaspirillum arcticum''<br /> | **[[1b8u]], [[1b8v]] – AaMDH+NAD - ''Aquaspirillum arcticum''<br /> | ||
**[[4i1i]] – LmMd + NAD – ''Leishmania major''<br /> | **[[4i1i]] – LmMd + NAD – ''Leishmania major''<br /> | ||
**[[5ujk]] – MeMd + NAD - ''Methylobacterium extorquens'' <br /> | **[[5ujk]] – MeMd + NAD - ''Methylobacterium extorquens'' <br /> | ||
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**[[4uup]] – Md + NADH - ''Trichiminad'' <br /> | **[[4uup]] – Md + NADH - ''Trichiminad'' <br /> | ||
**[[5kvv]] – MtMd + NADH – ''Mycobacterium tuberculosis'' <br /> | **[[5kvv]] – MtMd + NADH – ''Mycobacterium tuberculosis'' <br /> | ||
**[[6bal]] – HiMd + malate - Haemophilus influenzae<br /> | **[[6ihd]] – MsMd + NAD – ''Metallosphaera sedula''<br /> | ||
* Md binary complex | |||
**[[4wle]] – hMd2 + citrate <br /> | |||
**[[4wlf]] – hMd2 + malate <br /> | |||
**[[5kka]] – EcMd + inhibitor <br /> | |||
**[[5z3w]] – EcMd + Ag<br /> | |||
**[[2cmd]] - EcMd + citrate<br /> | |||
**[[3gvi]] - BmMDH+ADP<br /> | |||
**[[2hjr]] – MDH+adenosine diphosphoribose – ''Cryptosporidium parvum''<br /> | |||
**[[1bdm]] - TfMDH (mutant)+beta-6-hydroxy-1,4,5,6-tetrahydronicotinamide adenine dinucleotide<br /> | |||
**[[6bal]] – HiMd + malate - ''Haemophilus influenzae''<br /> | |||
**[[6qss]] – Md + Tb derivative – ''Ignicoccus islandicus''<br /> | |||
* Md ternary complex | * Md ternary complex | ||
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**[[4ros]] – MeMd + oxaloacetate + ADPR <br /> | **[[4ros]] – MeMd + oxaloacetate + ADPR <br /> | ||
**[[1emd]] – EcMd+NAD+citrate<br /> | **[[1emd]] – EcMd+NAD+citrate<br /> | ||
**[[6ihe]] – MsMd + NAD + malate <br /> | |||
**[[5zi2]] – yMd + NAD + ADP - yeast<br /> | |||
**[[5zi4]] – yMd + NAD + oxalacetate <br /> | |||
* apo-MDH | * apo-MDH | ||
**[[4wln]] – hMd2 <br /> | |||
**[[1mld]] – pMDH<br /> | |||
**[[5zi3]] – yMd <br /> | |||
**[[2j5r]], [[2j5k]], [[2j5q]], [[1d3a]], [[4jco]] – HmMDH <br /> | **[[2j5r]], [[2j5k]], [[2j5q]], [[1d3a]], [[4jco]] – HmMDH <br /> | ||
**[[2hlp]] – HmMDH (mutant)<br /> | **[[2hlp]] – HmMDH (mutant)<br /> | ||
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**[[1b8p]] – AaMDH <br /> | **[[1b8p]] – AaMDH <br /> | ||
**[[7mdh]] – MDH – Sorgum bicolor<br /> | **[[7mdh]] – MDH – Sorgum bicolor<br /> | ||
**[[3nep]] – Md – ''Salinibacter ruber''<br /> | **[[3nep]] – Md – ''Salinibacter ruber''<br /> | ||
**[[3p7m]] – Md – ''Francisella tularensis''<br /> | **[[3p7m]] – Md – ''Francisella tularensis''<br /> | ||