6rk2: Difference between revisions
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<StructureSection load='6rk2' size='340' side='right'caption='[[6rk2]], [[Resolution|resolution]] 2.09Å' scene=''> | <StructureSection load='6rk2' size='340' side='right'caption='[[6rk2]], [[Resolution|resolution]] 2.09Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6rk2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RK2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6rk2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens_a6 Agrobacterium tumefaciens a6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RK2 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6GR:2-(alpha-L-altropyranosyloxy)benzoic+acid'>6GR</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6GR:2-(alpha-L-altropyranosyloxy)benzoic+acid'>6GR</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sghA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1453998 Agrobacterium tumefaciens A6])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk2 OCA], [http://pdbe.org/6rk2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rk2 RCSB], [http://www.ebi.ac.uk/pdbsum/6rk2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk2 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk2 OCA], [http://pdbe.org/6rk2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rk2 RCSB], [http://www.ebi.ac.uk/pdbsum/6rk2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
It is highly intriguing how bacterial pathogens can quickly shut down energy-costly infection machinery once successful infection is established. This study depicts that mutation of repressor SghR increases the expression of hydrolase SghA in Agrobacterium tumefaciens, which releases plant defense signal salicylic acid (SA) from its storage form SA beta-glucoside (SAG). Addition of SA substantially reduces gene expression of bacterial virulence. Bacterial vir genes and sghA are differentially transcribed at early and later infection stages, respectively. Plant metabolite sucrose is a signal ligand that inactivates SghR and consequently induces sghA expression. Disruption of sghA leads to increased vir expression in planta and enhances tumor formation whereas mutation of sghR decreases vir expression and tumor formation. These results depict a remarkable mechanism by which A. tumefaciens taps on the reserved pool of plant signal SA to reprogram its virulence upon establishment of infection. | |||
Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals.,Wang C, Ye F, Chang C, Liu X, Wang J, Wang J, Yan XF, Fu Q, Zhou J, Chen S, Gao YG, Zhang LH Proc Natl Acad Sci U S A. 2019 Oct 11. pii: 1903695116. doi:, 10.1073/pnas.1903695116. PMID:31604827<ref>PMID:31604827</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6rk2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Agrobacterium tumefaciens a6]] | |||
[[Category: Beta-glucosidase]] | [[Category: Beta-glucosidase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
Revision as of 11:09, 23 October 2019
Complex structure of virulence factor SghA mutant with its substrate SAGComplex structure of virulence factor SghA mutant with its substrate SAG
Structural highlights
Publication Abstract from PubMedIt is highly intriguing how bacterial pathogens can quickly shut down energy-costly infection machinery once successful infection is established. This study depicts that mutation of repressor SghR increases the expression of hydrolase SghA in Agrobacterium tumefaciens, which releases plant defense signal salicylic acid (SA) from its storage form SA beta-glucoside (SAG). Addition of SA substantially reduces gene expression of bacterial virulence. Bacterial vir genes and sghA are differentially transcribed at early and later infection stages, respectively. Plant metabolite sucrose is a signal ligand that inactivates SghR and consequently induces sghA expression. Disruption of sghA leads to increased vir expression in planta and enhances tumor formation whereas mutation of sghR decreases vir expression and tumor formation. These results depict a remarkable mechanism by which A. tumefaciens taps on the reserved pool of plant signal SA to reprogram its virulence upon establishment of infection. Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals.,Wang C, Ye F, Chang C, Liu X, Wang J, Wang J, Yan XF, Fu Q, Zhou J, Chen S, Gao YG, Zhang LH Proc Natl Acad Sci U S A. 2019 Oct 11. pii: 1903695116. doi:, 10.1073/pnas.1903695116. PMID:31604827[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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