Luciferase: Difference between revisions
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== Relevance == | == Relevance == | ||
Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. | Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. | ||
== 3D Structures of Luciferase == | |||
[[Luciferase 3D structures]] | |||
</StructureSection> | </StructureSection> | ||
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**[[1luc]], [[1brl]] - VhLuc α+β chain<br /> | **[[1luc]], [[1brl]] - VhLuc α+β chain<br /> | ||
**[[1bsl]], [[1xkj]] - VhLuc β chain<br /> | **[[1bsl]], [[1xkj]] - VhLuc β chain<br /> | ||
**[[1vpr]] – Luc residues 866-1241 – ''Lingulodinium polyedrum''<br /> | |||
**[[6aaa]] – Luc - ''Amydetes''<br /> | |||
**[[6aah]], [[6ac3]] – Luc – ''Phrixothrix hirtus''<br /> | |||
**[[6fri]] – Luc β chain – ''Photobacterium leiognathi''<br /> | |||
*Luciferin 2-monooxygenase | *Luciferin 2-monooxygenase | ||
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**[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br /> | **[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br /> | ||
**[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br /> | **[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br /> | ||
**[[4g37]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br /> | **[[4g37]], [[6q2m]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br /> | ||
**[[5gz2]] – fLuc + thiazole derivative<br /> | **[[5gz2]] – fLuc + thiazole derivative<br /> | ||
**[[5gyz]] – fLuc + AMP + thiazole derivative<br /> | **[[5gyz]] – fLuc + AMP + thiazole derivative<br /> | ||