RNA Polymerase II: Difference between revisions
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This section will briefly discuss the chief structural components involved in the mechanism. | This section will briefly discuss the chief structural components involved in the mechanism. | ||
[[Image:Alpha-amanitin structure (1).png|alt text]] | |||
|The chemical structure of α-amanitin.]] Image:Alpha-amanitin structure (1).png | |||
[[Image:File.png|alt text]] | |||
[[ Image:RnapII struct features.jpg|300px|left|thumb| The clamp (magenta), wall (navy blue), rudder (red), bridge (orange), RNA (light blue), and DNA (blue) are depicted. See below for PDB's and residue numbers.]] | [[ Image:RnapII struct features.jpg|300px|left|thumb| The clamp (magenta), wall (navy blue), rudder (red), bridge (orange), RNA (light blue), and DNA (blue) are depicted. See below for PDB's and residue numbers.]] | ||
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α-Amanitin is a bicyclic octapeptide that adheres tightly with RNAP II, which blocks the elongation steps. α-amanitin binds in the funnel and interacts with the bridge helix and adjacent Rpb1, but it does not inhibit the RNA pol II’s interaction with NTP. Instead, α-amanitin likely challenges the bridge’s conformational change that is necessary for the purposed RNAP translocation step. α-Amanitin, found in the poisonous mushroom death cap, leads to death after several days. This time frame aligns with the rate at which mRNA’s and proteins turnover. [[Image:Alpha-amanitin structure (1).png | α-Amanitin is a bicyclic octapeptide that adheres tightly with RNAP II, which blocks the elongation steps. α-amanitin binds in the funnel and interacts with the bridge helix and adjacent Rpb1, but it does not inhibit the RNA pol II’s interaction with NTP. Instead, α-amanitin likely challenges the bridge’s conformational change that is necessary for the purposed RNAP translocation step. α-Amanitin, found in the poisonous mushroom death cap, leads to death after several days. This time frame aligns with the rate at which mRNA’s and proteins turnover. [[Image:Alpha-amanitin structure (1).png | ||
|The chemical structure of α-amanitin.]] Image:Alpha-amanitin structure (1).png | |The chemical structure of α-amanitin.]] Image:Alpha-amanitin structure (1).png | ||
[[Image:File.png|alt text]] | |||