2jzz: Difference between revisions

Revision as of 13:29, 30 April 2008

Solid-State NMR Structure of Microcrystalline Ubiquitin

OverviewOverview

Proton-driven 13C spin diffusion (PDSD) is a simple and robust two-dimensional NMR experiment. It leads to spectra with a high signal-to-noise ratio in which cross-peaks contain information about internuclear distances. We show that the total information content is sufficient to determine the atomic-resolution structure of a small protein from a single, uniformly 13C-, 15N-labeled microcrystalline sample. For the example of ubiquitin, the structure was determined by a manual procedure followed by an automatic optimization of the manual structure as well as by a fully automated structure determination approach. The relationship between internuclear distances and cross-peak intensities in the spectra is investigated.

About this StructureAbout this Structure

2JZZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy., Manolikas T, Herrmann T, Meier BH, J Am Chem Soc. 2008 Mar 26;130(12):3959-66. Epub 2008 Mar 6. PMID:18321098 Page seeded by OCA on Wed Apr 30 13:29:01 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2jzz.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2jzz |SIZE=350|CAPTION= <scene name='initialview01'>2jzz</scene>
+The line below this paragraph, containing "STRUCTURE_2jzz", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01803 Ubiquitin]</span>
+{{STRUCTURE_2jzz|  PDB=2jzz  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jzz OCA], [http://www.ebi.ac.uk/pdbsum/2jzz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jzz RCSB]</span>
-}}
 '''Solid-State NMR Structure of Microcrystalline Ubiquitin'''
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 [[Category: Manolikas, T.]]
 [[Category: Meier, B H.]]
-[[Category: cytoplasm]]
+[[Category: Cytoplasm]]
-[[Category: protein microcrystal]]
+[[Category: Protein microcrystal]]
-[[Category: signaling protein]]
+[[Category: Signaling protein]]
-[[Category: ubl conjugation]]
+[[Category: Ubl conjugation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:29:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:02:55 2008''