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==Crystal structure of the ferric enterobactin receptor (PfeA) mutant (R480A_Q482A) from Pseudomonas aeruginosa== | ==Crystal structure of the ferric enterobactin receptor (PfeA) mutant (R480A_Q482A) from Pseudomonas aeruginosa== | ||
<StructureSection load='5mzs' size='340' side='right' caption='[[5mzs]], [[Resolution|resolution]] 2.67Å' scene=''> | <StructureSection load='5mzs' size='340' side='right'caption='[[5mzs]], [[Resolution|resolution]] 2.67Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5mzs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MZS FirstGlance]. <br> | <table><tr><td colspan='2'>[[5mzs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MZS FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzs OCA], [http://pdbe.org/5mzs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mzs RCSB], [http://www.ebi.ac.uk/pdbsum/5mzs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzs ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pfeA, PA2688 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzs OCA], [http://pdbe.org/5mzs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mzs RCSB], [http://www.ebi.ac.uk/pdbsum/5mzs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzs ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/PFEA_PSEAE PFEA_PSEAE]] Specific receptor for the siderophore ferric enterobactin. | [[http://www.uniprot.org/uniprot/PFEA_PSEAE PFEA_PSEAE]] Specific receptor for the siderophore ferric enterobactin. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacteria use small molecules called siderophores to scavenge iron. Siderophore-Fe(3+) complexes are recognised by outer-membrane transporters and imported into the periplasm in a process dependent on the inner-membrane protein TonB. The siderophore enterobactin is secreted by members of the family Enterobacteriaceae, but many other bacteria including Pseudomonas species can use it. Here, we show that the Pseudomonas transporter PfeA recognises enterobactin using extracellular loops distant from the pore. The relevance of this site is supported by in vivo and in vitro analyses. We suggest there is a second binding site deeper inside the structure and propose that correlated changes in hydrogen bonds link binding-induced structural re-arrangements to the structural adjustment of the periplasmic TonB-binding motif. | |||
The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model.,Moynie L, Milenkovic S, Mislin GLA, Gasser V, Malloci G, Baco E, McCaughan RP, Page MGP, Schalk IJ, Ceccarelli M, Naismith JH Nat Commun. 2019 Aug 14;10(1):3673. doi: 10.1038/s41467-019-11508-y. PMID:31413254<ref>PMID:31413254</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5mzs" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ferric enterobactin receptor|Ferric enterobactin receptor]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Pseae]] | |||
[[Category: Moynie, L]] | [[Category: Moynie, L]] | ||
[[Category: Naismith, J H]] | [[Category: Naismith, J H]] | ||
Revision as of 18:48, 28 August 2019
Crystal structure of the ferric enterobactin receptor (PfeA) mutant (R480A_Q482A) from Pseudomonas aeruginosaCrystal structure of the ferric enterobactin receptor (PfeA) mutant (R480A_Q482A) from Pseudomonas aeruginosa
Structural highlights
Function[PFEA_PSEAE] Specific receptor for the siderophore ferric enterobactin. Publication Abstract from PubMedBacteria use small molecules called siderophores to scavenge iron. Siderophore-Fe(3+) complexes are recognised by outer-membrane transporters and imported into the periplasm in a process dependent on the inner-membrane protein TonB. The siderophore enterobactin is secreted by members of the family Enterobacteriaceae, but many other bacteria including Pseudomonas species can use it. Here, we show that the Pseudomonas transporter PfeA recognises enterobactin using extracellular loops distant from the pore. The relevance of this site is supported by in vivo and in vitro analyses. We suggest there is a second binding site deeper inside the structure and propose that correlated changes in hydrogen bonds link binding-induced structural re-arrangements to the structural adjustment of the periplasmic TonB-binding motif. The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model.,Moynie L, Milenkovic S, Mislin GLA, Gasser V, Malloci G, Baco E, McCaughan RP, Page MGP, Schalk IJ, Ceccarelli M, Naismith JH Nat Commun. 2019 Aug 14;10(1):3673. doi: 10.1038/s41467-019-11508-y. PMID:31413254[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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