Purine repressor: Difference between revisions

Revision as of 16:45, 20 August 2019

Function

Purine repressor (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a nucleotide co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides^[1].

Structural highlights

The . Water molecules are shown as red spheres. The DNA binding domain contains a and a hinge helix binding to to the DNA minor groove. ^[2].

Structure of E. coli purine processor complex with DNA and guanine (PDB entry 1wet)

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3D structures of purine repressor3D structures of purine repressor

Updated on 20-August-2019

ReferencesReferences

↑ Wilson HR, Turnbough CL Jr. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12. J Bacteriol. 1990 Jun;172(6):3208-13. PMID:1971621
↑ Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem. 1997 Sep 5;272(36):22648-53. PMID:9278422

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] Wilson HR, Turnbough CL Jr. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12. J Bacteriol. 1990 Jun;172(6):3208-13. PMID:1971621

[2] Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem. 1997 Sep 5;272(36):22648-53. PMID:9278422

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[2]

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 == Structural highlights ==
-The <scene name='55/554904/Cv/2'>PurP guanine co-repressor binding site includes stacking interactions as well as hydrogen bonded ones</scene>. Water molecules shown as red spheres. The DNA binding domain contains a <scene name='55/554904/Cv/3'>helix-turn-helix-loop-helix motif which interacts with the DNA major groove</scene> and a hinge helix binding to to the DNA minor groove.  <scene name='55/554904/Cv/4'>Residue L54 interdigitates with the DNA central base pair</scene><ref>PMID:9278422</ref>.
+The <scene name='55/554904/Cv/5'>PurP guanine co-repressor binding site includes stacking interactions as well as hydrogen bonded ones</scene>. Water molecules are shown as red spheres. The DNA binding domain contains a <scene name='55/554904/Cv/6'>helix-turn-helix-loop-helix motif which interacts with the DNA major groove</scene> and a hinge helix binding to to the DNA minor groove. <scene name='55/554904/Cv/7'>Residue L54 interdigitates with the DNA central base pair</scene><ref>PMID:9278422</ref>.
 </StructureSection>