3ru8: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab== | ==Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab== | ||
<StructureSection load='3ru8' size='340' side='right' caption='[[3ru8]], [[Resolution|resolution]] 2.07Å' scene=''> | <StructureSection load='3ru8' size='340' side='right'caption='[[3ru8]], [[Resolution|resolution]] 2.07Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3ru8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aids_virus Aids virus] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RU8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3ru8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aids_virus Aids virus] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RU8 FirstGlance]. <br> | ||
| Line 18: | Line 18: | ||
==See Also== | ==See Also== | ||
*[[3D structures | *[[Antibody 3D structures|Antibody 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 25: | Line 25: | ||
[[Category: Aids virus]] | [[Category: Aids virus]] | ||
[[Category: Human]] | [[Category: Human]] | ||
[[Category: Large Structures]] | |||
[[Category: Carrico, C T.D]] | [[Category: Carrico, C T.D]] | ||
[[Category: Strong, R K]] | [[Category: Strong, R K]] | ||
Revision as of 21:02, 14 August 2019
Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 FabStructure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab
Structural highlights
Publication Abstract from PubMedThe manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting. Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.,Azoitei ML, Correia BE, Ban YE, Carrico C, Kalyuzhniy O, Chen L, Schroeter A, Huang PS, McLellan JS, Kwong PD, Baker D, Strong RK, Schief WR Science. 2011 Oct 21;334(6054):373-6. PMID:22021856[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||