6fbe: Difference between revisions
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==KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the third primer nucleotide.== | ==KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the third primer nucleotide.== | ||
<StructureSection load='6fbe' size='340' side='right' caption='[[6fbe]], [[Resolution|resolution]] 1.59Å' scene=''> | <StructureSection load='6fbe' size='340' side='right'caption='[[6fbe]], [[Resolution|resolution]] 1.59Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6fbe]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FBE FirstGlance]. <br> | <table><tr><td colspan='2'>[[6fbe]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FBE FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 6fbe" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6fbe" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Atcc 25104]] | [[Category: Atcc 25104]] | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Large Structures]] | |||
[[Category: Diederichs, K]] | [[Category: Diederichs, K]] | ||
[[Category: Kropp, H M]] | [[Category: Kropp, H M]] | ||
Revision as of 20:18, 14 August 2019
KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the third primer nucleotide.KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the third primer nucleotide.
Structural highlights
Publication Abstract from PubMedDNA polymerases have evolved to process the four canonical nucleotides accurately. Nevertheless, these enzymes are also known to process modified nucleotides, which is the key to numerous core biotechnology applications. Processing of modified nucleotides includes incorporation of the modified nucleotide and postincorporation elongation to proceed with the synthesis of the nascent DNA strand. The structural basis for postincorporation elongation is currently unknown. We addressed this issue and successfully crystallized KlenTaq DNA polymerase in six closed ternary complexes containing the enzyme, the modified DNA substrate, and the incoming nucleotide. Each structure shows a high-resolution snapshot of the elongation of a modified primer, where the modification "moves" from the 3'-primer terminus upstream to the sixth nucleotide in the primer strand. Combining these data with quantum mechanics/molecular mechanics calculations and biochemical studies elucidates how the enzyme and the modified substrate mutually modulate their conformations without compromising the enzyme's activity significantly. The study highlights the plasticity of the system as origin of the broad substrate properties of DNA polymerases and facilitates the design of improved systems. Snapshots of a modified nucleotide moving through the confines of a DNA polymerase.,Kropp HM, Durr SL, Peter C, Diederichs K, Marx A Proc Natl Acad Sci U S A. 2018 Sep 17. pii: 1811518115. doi:, 10.1073/pnas.1811518115. PMID:30224478[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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