2ffl: Difference between revisions
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==Crystal Structure of Dicer from Giardia intestinalis== | ==Crystal Structure of Dicer from Giardia intestinalis== | ||
<StructureSection load='2ffl' size='340' side='right' caption='[[2ffl]], [[Resolution|resolution]] 3.33Å' scene=''> | <StructureSection load='2ffl' size='340' side='right'caption='[[2ffl]], [[Resolution|resolution]] 3.33Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2ffl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giain Giain]. The February 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Small Interfering RNA'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_2 10.2210/rcsb_pdb/mom_2008_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FFL FirstGlance]. <br> | <table><tr><td colspan='2'>[[2ffl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giain Giain]. The February 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Small Interfering RNA'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_2 10.2210/rcsb_pdb/mom_2008_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FFL FirstGlance]. <br> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Giain]] | [[Category: Giain]] | ||
[[Category: Large Structures]] | |||
[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
[[Category: Ribonuclease III]] | [[Category: Ribonuclease III]] | ||
Revision as of 11:59, 24 July 2019
Crystal Structure of Dicer from Giardia intestinalisCrystal Structure of Dicer from Giardia intestinalis
Structural highlights
Publication Abstract from PubMedThe specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end. Structural basis for double-stranded RNA processing by Dicer.,Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA Science. 2006 Jan 13;311(5758):195-8. PMID:16410517[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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