6jtd: Difference between revisions
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<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution|resolution]] 1.85Å' scene=''> | <StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JTD FirstGlance]. <br> | <table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trollius_chinensis Trollius chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JTD FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [http://pdbe.org/6jtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [http://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [http://pdbe.org/6jtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [http://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr> | ||
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</div> | </div> | ||
<div class="pdbe-citations 6jtd" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6jtd" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Trollius chinensis]] | |||
[[Category: Yun, C H]] | [[Category: Yun, C H]] | ||
[[Category: Zhao, P]] | [[Category: Zhao, P]] | ||
Revision as of 11:37, 24 July 2019
Crystal structure of TcCGT1 in complex with UDPCrystal structure of TcCGT1 in complex with UDP
Structural highlights
Publication Abstract from PubMedIn this work, we explored the catalytic promiscuity of TcCGT1, a new C-glycosyltransferase (CGT) from the medicinal plant Trollius chinensis. TcCGT1 could efficiently and regio-specifically catalyze 8-C-glycosylation of 36 flavones and other flavonoids, and could also catalyze the O-glycosylation of diverse phenolics. Moreover, the crystal structure of TcCGT1 in complex with uridine diphosphate was determined at 1.85 A resolution. Structural analysis with molecular docking revealed a new model for catalytic mechanism of TcCGT1, which was initiated by substrate spontaneous deprotonation. The spacious binding pocket explains the robust substrate promiscuity, and binding pose of the substrate determines C- or O-glycosylation activity. Site-directed mutagenesis at two residues (I94E and G284K) switched C- to O-glycosylation. This work highlights TcCGT1 as the first plant CGT with a crystal structure and the first flavone 8-C-glycosyltransferase, and provides a basis for protein engineering to design efficient glycosylation biocatalysts for drug discovery. Molecular Characterization and Structural Basis of a Promiscuous C-Glycosyltransferase from Trollius chinensis.,He JB, Zhao P, Hu ZM, Liu S, Kuang Y, Zhang M, Li B, Yun CH, Qiao X, Ye M Angew Chem Int Ed Engl. 2019 Jun 4. doi: 10.1002/anie.201905505. PMID:31163097[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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