4z0c: Difference between revisions
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==Crystal structure of TLR13-ssRNA13 complex== | ==Crystal structure of TLR13-ssRNA13 complex== | ||
<StructureSection load='4z0c' size='340' side='right' caption='[[4z0c]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='4z0c' size='340' side='right'caption='[[4z0c]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4z0c]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z0C FirstGlance]. <br> | <table><tr><td colspan='2'>[[4z0c]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z0C FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z0c OCA], [http://pdbe.org/4z0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z0c RCSB], [http://www.ebi.ac.uk/pdbsum/4z0c PDBsum]</span></td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tlr13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z0c OCA], [http://pdbe.org/4z0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z0c RCSB], [http://www.ebi.ac.uk/pdbsum/4z0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z0c ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 4z0c" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4z0c" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Toll-like Receptors|Toll-like Receptors]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Lk3 transgenic mice]] | |||
[[Category: Chai, J]] | [[Category: Chai, J]] | ||
[[Category: Han, Z]] | [[Category: Han, Z]] | ||
Revision as of 10:24, 19 June 2019
Crystal structure of TLR13-ssRNA13 complexCrystal structure of TLR13-ssRNA13 complex
Structural highlights
Function[TLR13_MOUSE] Component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Specifically binds the 5'-CGGAAAGACC-3' sequence on bacterial 23S rRNA, a sequence also bound by MLS group antibiotics (including erythromycin). May also recognize vesicular stomatitis virus; however, these data require additional evidences.[1] [2] [3] Publication Abstract from PubMedToll-like receptors (TLRs) have crucial roles in innate immunity, functioning as pattern-recognition receptors. TLR13 recognizes a conserved sequence from bacterial 23S rRNA and then triggers an immune response. Here we report the crystal structure of the mouse TLR13 ectodomain bound by a 13-nt single-stranded (ss) RNA derived from 23S rRNA. The ssRNA induces TLR13 dimerization but assumes a stem-loop-like structure that is completely different from that in the bacterial ribosome but nevertheless is crucial for TLR13 recognition. Most of the RNA nucleotides are splayed out to make base-specific contacts with the concave surface of TLR13, and RNA-specific interactions are important to allow TLR13 to distinguish RNA from DNA. Interestingly, a viral-derived 16-nt ssRNA predicted to form a similar stem-loop-like structure also induces TLR13 activation. Together, our results reveal the structural mechanism of TLR13's sequence- and conformation-specific recognition of ssRNA. Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13.,Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D, Wang J, Wang HW, Chai J Nat Struct Mol Biol. 2015 Oct;22(10):782-787. doi: 10.1038/nsmb.3080. Epub 2015, Aug 31. PMID:26323037[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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