2j5m: Difference between revisions
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[[Category: pyrrolidone carboxylic acid]] | [[Category: pyrrolidone carboxylic acid]] | ||
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Revision as of 19:20, 5 November 2007
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STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0
OverviewOverview
We have determined the crystal structure of the chloroperoxidase (CPO), hydroperoxo reaction intermediate (CPO compound 0) at 1.75-A resolution., The intermediate was generated through controlled photoreduction of the, CPO oxygen complex during x-ray data collection, which was monitored by, recording of the crystal absorption spectra. Initially, the peroxo-anion, species was formed and then protonated to yield compound 0. Quantum, chemical calculations indicate that the peroxo-anion species is not stable, and collapses instantaneously to compound 0. Compound 0 is present in the, ferric low-spin doublet ground state and is characterized by a long O O, bond length of 1.5 A and a Fe O bond distance of 1.8 A, which is also, observed in the crystal structure.
About this StructureAbout this Structure
2J5M is a Single protein structure of sequence from Leptoxyphium fumago with NAG, MAN, MN, ACT, HEM, PEO and EDO as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
Structure and quantum chemical characterization of chloroperoxidase compound 0, a common reaction intermediate of diverse heme enzymes., Kuhnel K, Derat E, Terner J, Shaik S, Schlichting I, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):99-104. Epub 2006 Dec 26. PMID:17190816
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