6qvk: Difference between revisions
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The | ==The cryo-EM structure of bacteriophage phi29 prohead== | ||
<StructureSection load='6qvk' size='340' side='right'caption='[[6qvk]], [[Resolution|resolution]] 3.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6qvk]] is a 400 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_phi29 Bacillus phage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QVK FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qvk OCA], [http://pdbe.org/6qvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qvk RCSB], [http://www.ebi.ac.uk/pdbsum/6qvk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qvk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/CAPSD_BPPH2 CAPSD_BPPH2]] Assembles to form a prolate capsid shell of about 54 nm in length and 45 nm in width, with a T=3, Q=5 symmetry.<ref>PMID:15837419</ref> <ref>PMID:17098197</ref> <ref>PMID:9814712</ref> [[http://www.uniprot.org/uniprot/CAPSF_BPPH2 CAPSF_BPPH2]] Protein that forms the 55 capsid fibers. These fibers are not always present and may have been lost in some lab strains. They may enhance the attachment of the virions onto the host cell wall.<ref>PMID:409854</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The mature virion of the tailed bacteriophage varphi29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile tail. Here, we report the near-atomic structures of the varphi29 pre-genome packaging head (prohead), the mature virion and the genome-emptied virion. Structural comparisons suggest local rotation or oscillation of the head-tail connector upon DNA packaging and release. Termination of the DNA packaging occurs through pressure-dependent correlative positional and conformational changes in the connector. The funnel-shaped tail lower collar attaches the expanded narrow end of the connector and has a 180-A long, 24-strand beta barrel narrow stem tube that undergoes conformational changes upon genome release. The appendages form an interlocked assembly attaching the tail around the collar. The membrane active long loops at the distal end of the tail knob exit during the late stage of infection and form the cone-shaped tip of a largely hydrophobic helix barrel, prepared for membrane penetration. | |||
Structural assembly of the tailed bacteriophage varphi29.,Xu J, Wang D, Gui M, Xiang Y Nat Commun. 2019 May 30;10(1):2366. doi: 10.1038/s41467-019-10272-3. PMID:31147544<ref>PMID:31147544</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6qvk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus phage phi29]] | |||
[[Category: Large Structures]] | |||
[[Category: Gui, M]] | [[Category: Gui, M]] | ||
[[Category: Xiang, Y]] | |||
[[Category: Xu, J]] | [[Category: Xu, J]] | ||
[[Category: | [[Category: Bacteriophage]] | ||
[[Category: Phi29]] | |||
[[Category: Prohead]] | |||
[[Category: Virus]] | |||
Revision as of 08:49, 12 June 2019
The cryo-EM structure of bacteriophage phi29 proheadThe cryo-EM structure of bacteriophage phi29 prohead
Structural highlights
Function[CAPSD_BPPH2] Assembles to form a prolate capsid shell of about 54 nm in length and 45 nm in width, with a T=3, Q=5 symmetry.[1] [2] [3] [CAPSF_BPPH2] Protein that forms the 55 capsid fibers. These fibers are not always present and may have been lost in some lab strains. They may enhance the attachment of the virions onto the host cell wall.[4] Publication Abstract from PubMedThe mature virion of the tailed bacteriophage varphi29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile tail. Here, we report the near-atomic structures of the varphi29 pre-genome packaging head (prohead), the mature virion and the genome-emptied virion. Structural comparisons suggest local rotation or oscillation of the head-tail connector upon DNA packaging and release. Termination of the DNA packaging occurs through pressure-dependent correlative positional and conformational changes in the connector. The funnel-shaped tail lower collar attaches the expanded narrow end of the connector and has a 180-A long, 24-strand beta barrel narrow stem tube that undergoes conformational changes upon genome release. The appendages form an interlocked assembly attaching the tail around the collar. The membrane active long loops at the distal end of the tail knob exit during the late stage of infection and form the cone-shaped tip of a largely hydrophobic helix barrel, prepared for membrane penetration. Structural assembly of the tailed bacteriophage varphi29.,Xu J, Wang D, Gui M, Xiang Y Nat Commun. 2019 May 30;10(1):2366. doi: 10.1038/s41467-019-10272-3. PMID:31147544[5] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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