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==Crystal structure of sulfoacetaldehyde reductase from Bifidobacterium kashiwanohense in complex with NAD+== | |||
<StructureSection load='6jkp' size='340' side='right'caption='[[6jkp]], [[Resolution|resolution]] 3.01Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6jkp]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JKP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JKP FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jkp OCA], [http://pdbe.org/6jkp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jkp RCSB], [http://www.ebi.ac.uk/pdbsum/6jkp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jkp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hydroxyethylsulfonate (isethionate) present in mammalian tissue is thought to be derived from aminoethylsulfonate (taurine), as a byproduct of taurine nitrogen assimilation by certain anaerobic bacteria inhabiting the taurine-rich mammalian gut. In previously studied pathways occurring in environmental bacteria, isethionate is generated by the enzyme sulfoacetaldehyde reductase IsfD, belonging to the short-chain dehydrogenase / reductase (SDR) family. An unrelated sulfoacetaldehyde reductase SarD, belonging to the metal-dependent alcohol dehydrogenase superfamily (M-ADH), was recently discovered in the human gut sulfite-reducing bacterium Bilophila wadsworthia ( Bw SarD). Here we report the structural and biochemical characterization of a sulfoacetaldehyde reductase from the human gut fermenting bacterium Bifidobacterium kashiwanohense ( Bk TauF). Bk TauF belongs to the M-ADH family, but is distantly related to Bw SarD (28% sequence identity). The crystal structures of Bk TauF in the apo form and in a binary complex with NAD(+) were determined at 1.9 and 3.0 A resolution respectively. Mutagenesis studies were carried out to investigate the involvement of active site residues in binding the sulfonate substrate. Our studies demonstrate the presence of sulfoacetaldehyde reductase in Bifidobacteria, with a possible role in isethionate production as a byproduct of taurine nitrogen assimilation. | |||
Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense.,Zhou Y, Wei Y, Nanjaraj Urs AN, Lin L, Xu T, Hu Y, Ang EL, Zhao H, Yuchi Z, Zhang Y Biosci Rep. 2019 May 23. pii: BSR20190715. doi: 10.1042/BSR20190715. PMID:31123167<ref>PMID:31123167</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 6jkp" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Lin, L]] | [[Category: Lin, L]] | ||
[[Category: Xu, T]] | |||
[[Category: Yuchi, Z]] | [[Category: Yuchi, Z]] | ||
[[Category: | [[Category: Zhang, Y]] | ||
[[Category: Zhou, Y]] | [[Category: Zhou, Y]] | ||
[[Category: Oxidoreductase]] | |||
[[Category: Sulfoacetaldehyde reductase nadh]] | |||
Revision as of 08:36, 12 June 2019
Crystal structure of sulfoacetaldehyde reductase from Bifidobacterium kashiwanohense in complex with NAD+Crystal structure of sulfoacetaldehyde reductase from Bifidobacterium kashiwanohense in complex with NAD+
Structural highlights
Publication Abstract from PubMedHydroxyethylsulfonate (isethionate) present in mammalian tissue is thought to be derived from aminoethylsulfonate (taurine), as a byproduct of taurine nitrogen assimilation by certain anaerobic bacteria inhabiting the taurine-rich mammalian gut. In previously studied pathways occurring in environmental bacteria, isethionate is generated by the enzyme sulfoacetaldehyde reductase IsfD, belonging to the short-chain dehydrogenase / reductase (SDR) family. An unrelated sulfoacetaldehyde reductase SarD, belonging to the metal-dependent alcohol dehydrogenase superfamily (M-ADH), was recently discovered in the human gut sulfite-reducing bacterium Bilophila wadsworthia ( Bw SarD). Here we report the structural and biochemical characterization of a sulfoacetaldehyde reductase from the human gut fermenting bacterium Bifidobacterium kashiwanohense ( Bk TauF). Bk TauF belongs to the M-ADH family, but is distantly related to Bw SarD (28% sequence identity). The crystal structures of Bk TauF in the apo form and in a binary complex with NAD(+) were determined at 1.9 and 3.0 A resolution respectively. Mutagenesis studies were carried out to investigate the involvement of active site residues in binding the sulfonate substrate. Our studies demonstrate the presence of sulfoacetaldehyde reductase in Bifidobacteria, with a possible role in isethionate production as a byproduct of taurine nitrogen assimilation. Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense.,Zhou Y, Wei Y, Nanjaraj Urs AN, Lin L, Xu T, Hu Y, Ang EL, Zhao H, Yuchi Z, Zhang Y Biosci Rep. 2019 May 23. pii: BSR20190715. doi: 10.1042/BSR20190715. PMID:31123167[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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