6e4v: Difference between revisions
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<StructureSection load='6e4v' size='340' side='right'caption='[[6e4v]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='6e4v' size='340' side='right'caption='[[6e4v]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6e4v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E4V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E4V FirstGlance]. <br> | <table><tr><td colspan='2'>[[6e4v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bw25113 Escherichia coli bw25113]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E4V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E4V FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=HWS:COPROGEN'>HWS</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=HWS:COPROGEN'>HWS</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fhuE, b1102, JW1088 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=679895 Escherichia coli BW25113])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e4v OCA], [http://pdbe.org/6e4v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e4v RCSB], [http://www.ebi.ac.uk/pdbsum/6e4v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e4v ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e4v OCA], [http://pdbe.org/6e4v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e4v RCSB], [http://www.ebi.ac.uk/pdbsum/6e4v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e4v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/FHUE_ECOLI FHUE_ECOLI]] Required for the uptake of Fe(3+) via coprogen, ferrioxamine B, and rhodotorulic acid. | [[http://www.uniprot.org/uniprot/FHUE_ECOLI FHUE_ECOLI]] Required for the uptake of Fe(3+) via coprogen, ferrioxamine B, and rhodotorulic acid. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In order to survive in mixed microbial communities, some species of fungi secrete coprogens, siderophores that facilitate capture of the scarce nutrient iron. The TonB-dependent transporter FhuE is integrated in the outer membrane of Gram-negative bacteria and has been reported to scavenge these fungally produced coprogens. In this work, an Escherichia coli strain was engineered that is dependent solely on FhuE for its access to siderophore-sequestered iron. Using this tool, it is shown that while FhuE is highly active in the import of coprogens, it has some level of promiscuity, acting as a low-affinity transporter for related siderophores. The crystal structure of FhuE in complex with coprogen was determined, providing a structural basis to explain this selective promiscuity. The structural data, in combination with functional analysis, presented in this work show that FhuE has evolved to specifically engage with planar siderophores. A potential evolutionary driver, and a critical consequence of this selectivity, is that it allows FhuE to exclude antibiotics that mimic nonplanar hydroxamate siderophores: these toxic molecules could otherwise cross the outer membrane barrier through a Trojan horse mechanism. | |||
Determination of the molecular basis for coprogen import by Gram-negative bacteria.,Grinter R, Lithgow T IUCrJ. 2019 Apr 5;6(Pt 3):401-411. doi: 10.1107/S2052252519002926. eCollection, 2019 May 1. PMID:31098021<ref>PMID:31098021</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6e4v" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli bw25113]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Grinter, R]] | [[Category: Grinter, R]] | ||
Revision as of 09:32, 29 May 2019
The Crystal Structure of FhuE from E. coli in complex with its substrate CoprogenThe Crystal Structure of FhuE from E. coli in complex with its substrate Coprogen
Structural highlights
Function[FHUE_ECOLI] Required for the uptake of Fe(3+) via coprogen, ferrioxamine B, and rhodotorulic acid. Publication Abstract from PubMedIn order to survive in mixed microbial communities, some species of fungi secrete coprogens, siderophores that facilitate capture of the scarce nutrient iron. The TonB-dependent transporter FhuE is integrated in the outer membrane of Gram-negative bacteria and has been reported to scavenge these fungally produced coprogens. In this work, an Escherichia coli strain was engineered that is dependent solely on FhuE for its access to siderophore-sequestered iron. Using this tool, it is shown that while FhuE is highly active in the import of coprogens, it has some level of promiscuity, acting as a low-affinity transporter for related siderophores. The crystal structure of FhuE in complex with coprogen was determined, providing a structural basis to explain this selective promiscuity. The structural data, in combination with functional analysis, presented in this work show that FhuE has evolved to specifically engage with planar siderophores. A potential evolutionary driver, and a critical consequence of this selectivity, is that it allows FhuE to exclude antibiotics that mimic nonplanar hydroxamate siderophores: these toxic molecules could otherwise cross the outer membrane barrier through a Trojan horse mechanism. Determination of the molecular basis for coprogen import by Gram-negative bacteria.,Grinter R, Lithgow T IUCrJ. 2019 Apr 5;6(Pt 3):401-411. doi: 10.1107/S2052252519002926. eCollection, 2019 May 1. PMID:31098021[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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