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==Structure of Herceptin Fab without antigen== | |||
<StructureSection load='6mh2' size='340' side='right'caption='[[6mh2]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6mh2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MH2 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mh2 OCA], [http://pdbe.org/6mh2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mh2 RCSB], [http://www.ebi.ac.uk/pdbsum/6mh2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mh2 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We developed a repertoire approach to generate human antibody bispecifics. Using phage display selection of antibody heavy chains in the presence of a competitor light chain and providing a cognate light chain with an affinity handle, we identified mutations that prevent heavy/light chain mispairing. The strategy allows for the selection of human antibody chains that autonomously assemble into bispecifics. | |||
Human Antibody Bispecifics through Phage Display Selection.,Luthra A, Langley DB, Schofield P, Jackson J, Abdelatti M, Rouet R, Nevoltris D, Mazigi O, Crossett B, Christie M, Christ D Biochemistry. 2019 Apr 2;58(13):1701-1704. doi: 10.1021/acs.biochem.9b00037. Epub, 2019 Mar 22. PMID:30900875<ref>PMID:30900875</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6mh2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Christ, D]] | |||
[[Category: Christie, M]] | |||
[[Category: Langley, D B]] | |||
[[Category: Luthra, A]] | |||
[[Category: Antibody]] | |||
[[Category: Fab fragment]] | |||
[[Category: Herceptin]] | |||
[[Category: Immune system]] | |||
Revision as of 09:13, 29 May 2019
Structure of Herceptin Fab without antigenStructure of Herceptin Fab without antigen
Structural highlights
Publication Abstract from PubMedWe developed a repertoire approach to generate human antibody bispecifics. Using phage display selection of antibody heavy chains in the presence of a competitor light chain and providing a cognate light chain with an affinity handle, we identified mutations that prevent heavy/light chain mispairing. The strategy allows for the selection of human antibody chains that autonomously assemble into bispecifics. Human Antibody Bispecifics through Phage Display Selection.,Luthra A, Langley DB, Schofield P, Jackson J, Abdelatti M, Rouet R, Nevoltris D, Mazigi O, Crossett B, Christie M, Christ D Biochemistry. 2019 Apr 2;58(13):1701-1704. doi: 10.1021/acs.biochem.9b00037. Epub, 2019 Mar 22. PMID:30900875[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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